Biotin anti-human CD11b (activated) Antibody

Pricing & Availability
Clone
CBRM1/5 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM
Isotype
Mouse IgG1, κ
CBRM15_Biotin_CD11b_Antibody_092017
PMA-activated (10 minutes) human peripheral blood granulocytes were stained with CD11b (clone CBRM1/5) Biotin (filled histogram) or mouse IgG1, κ Biotin isotype control (open histogram)followed by Streptavidin PE.
  • CBRM15_Biotin_CD11b_Antibody_092017
    PMA-activated (10 minutes) human peripheral blood granulocytes were stained with CD11b (clone CBRM1/5) Biotin (filled histogram) or mouse IgG1, κ Biotin isotype control (open histogram)followed by Streptavidin PE.
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301416 100 µg $258.00
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Description

The CBRM1/5 antibody reacts with an activated form of human CD11b, a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils as well as adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen and factor X.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The CBRM1/5 antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol-esters. This antibody does not recognize "non-activated" CD11b. The epitope recognized by CBRM1/5 is contained in the I domain of the a chain. Clone CBRM1/5 binds the I domain, close to the ligand binding site. It recognizes a conformational change of the integrin9.

Additional reported applications (for the relevant formats) include: immunoprecipitation, and blocking cell adhesion to fibrinogen and ICAM-11. The CBRM1/5 antibody binds specifically to the activated form of CD11b. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301408).

Application References

(PubMed link indicates BioLegend citation)
  1. Diamond M, et al. 1993. J. Cell Biol. 120:545. (Block)
  2. Bryn T, et al. 2006. J. Immunol. 176:7361. PubMed
  3. Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200.
  4. Barthel SR,et al.2006. Am J. Respir Cell Mol Biol.35:378. PubMed
  5. Pillay J,et al. 2010. J. Leukocyte Biol. 88:211. PubMed
  6. Wilson RP, et al. 2011. Infect Immun. 79:830. PubMed
  7. Koleva RI, et al. 2012. Blood. 119:4878. PubMed.
  8. Eleftherious D, et al. 2012. Neurology. 79:2089. PubMed
  9. Oxvig C, et al. 1999. Proc. Natl. Acad. Sci. USA 96:2215.
  10. Muller-Edenborn B, et al. 2014. Br J Anaesth. PubMed
  11. Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
RRID
AB_2715799 (BioLegend Cat. No. 301416)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 165-170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, T cell subset, B cell subset

Function
Adhesion, phagocytosis, chemotaxis, neutrophil activation
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Stewart M, et al. 1995. Curr. Opin. Cell Biol. 7:690.
2. Petty H, et al. 1996. Immunol. Today 17:209.

Gene ID
3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org
Go To Top Version: 0    Revision Date: 09/20/2017

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