Biotin anti-human CD25 Antibody

Pricing & Availability
Clone
S20019D (See other available formats)
Regulatory Status
RUO
Other Names
interleukin-2 receptor alpha chain, interluekin-2 receptor, IL2RA, IL-2Rα
Isotype
Mouse IgG1, κ
A_S20019D_Biotin_CD25_Antibody_2_071624
Human peripheral blood lymphocytes were stained with anti-human CD4 (clone RPA-T4) Pacific Blue™ and biotinylated anti-human CD25 (clone S20019D) (left) or biotinylated mouse IgG1, κ isotype control (right), followed by Streptavidin PE.
  • A_S20019D_Biotin_CD25_Antibody_2_071624
    Human peripheral blood lymphocytes were stained with anti-human CD4 (clone RPA-T4) Pacific Blue™ and biotinylated anti-human CD25 (clone S20019D) (left) or biotinylated mouse IgG1, κ isotype control (right), followed by Streptavidin PE.
  • B_S20019D_Biotin_CD25_Antibody_1_071624
    PHA-activated (3 days) human peripheral blood mononuclear cells were stained with biotinylated anti-human CD25 (clone S20019D) (filled histogram) or biotinylated mouse IgG1, κ isotype control (open histogram), followed by Streptavidin PE. Data shown was gated on the lymphocyte population.
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Cat # Size Price Quantity Check Availability
385225 50 µg $130.00
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Description

CD25 is a 55 kD transmembrane protein also known as interleukin-2 receptor alpha chain. CD25 in combination with CD122 and CD132 form a high affinity IL-2 receptor that binds to IL-2, playing a crucial role in activation of JAK1/JAK3 and STAT5 signaling. It is expressed on activated T cells as well as on CD4+ T regulatory cells, some progenitor B-cells and activated monocytes. 

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD25 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone S20019D can block binding of clone M-A251 on target cells and is blocked by M-A251.

Clone S20019D does not block binding of clones S20019C, BC96, and TÜ69 on target cells.

Clone S20019D does not affect binding of IL-2 to the receptor. 

Clone S20019D shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.

Antigen Details

Structure
55 kD transmembrane protein
Distribution

Activated T cells, T regulatory cells, monocytes

Function
IL-2 receptor
Interaction
CD122, CD132
Ligand/Receptor
IL-2
Cell Type
Lymphocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Waldmann T. 1991. J Biol Chem. 266:2681-4.
  3. Sakaguchi S, et al. 1995. The Journal of Immunology. 155:1151-64.
Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org
Go To Top Version: 1    Revision Date: 07/16/2024

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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