Biotin anti-human CD40 Antibody

Pricing & Availability
Clone
5C3 (See other available formats)
Regulatory Status
RUO
Workshop
V CD40.4
Other Names
BP50, TNFRSF5
Isotype
Mouse IgG1, κ
5C3_Biotin_CD40_Antibody_120315
Human peripheral blood lymphocytes were stained with biotinylated CD40 (clone 5C3, filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by SAV-PE.
  • 5C3_Biotin_CD40_Antibody_120315
    Human peripheral blood lymphocytes were stained with biotinylated CD40 (clone 5C3, filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by SAV-PE.
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334343 100 µg $141.00
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Description

CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The 5C3 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially blocking CD40 binding to CD40L, and B cells rescue from apoptosis.

Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
Baboon, Chimpanzee, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation1, partial inhibition of CD40 binding to CD40L3, and B cell rescue from apoptosis1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman SF, et al. 1995. ed. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
  2. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  3. Pound JD, et al. 1999. Int. Immunol. 11:11.
  4. Shey MS, et al. 2014. J Immunol. 192:4833. PubMed
  5. Sondergaard JN, et al. 2014. Mol Immunol. 59:180. PubMed
  6. Saiki O, et al. 1995. J Clin. Invest. 2: 510-4 (Costim)
RRID
AB_2566580 (BioLegend Cat. No. 334343)

Antigen Details

Structure
TNFR superfamily, type I glycoprotein, 48 kD
Distribution

B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts

Function
B cell differentiation, costimulation, isotype class-switching, rescues B cells from apoptosis
Ligand/Receptor
CD154 (CD40 ligand)
Cell Type
B cells, Dendritic cells, Endothelial cells, Fibroblasts, Macrophages
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881.
2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591.

Gene ID
958 View all products for this Gene ID
UniProt
View information about CD40 on UniProt.org
Go To Top Version: 1    Revision Date: 12/07/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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