- Clone
- JKFb-2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Fibroblast Growth Factor-basic, bFGF, FGF-2, Heparin-binding growth factor
- Isotype
- Mouse IgG1, κ
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 508702 | 500 µg | $417.00 | |||
Fibroblast growth factor-basic (FGF-b, FGF-2) is a heparin-binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-basic also exerts a potent angiogenic activity in vivo. FGF-basic has been isolated from neural, pituitary, adrenal cortex, and placental tissues. The JKFb-2 antibody reacts with human fibroblast growth factor - basic (FGF-basic).
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human bFGF
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection
- Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human FGF-basic protein ranging from 500 to 7.8 pg/ml are recommended for each ELISA plate.It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA Detection: The biotinylated JKFb-2 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified BHFB15 antibody (Cat. No. 514801/514802) as the capture antibody.
- RRID
-
AB_315527 (BioLegend Cat. No. 508702)
Antigen Details
- Structure
- FGF; 17.2 kD (Mammalian)
- Bioactivity
- Modulator of cell proliferation, motility, differentiation, angiogenesis; affinity for heparin; associated with ECM
- Cell Sources
- Brain, retina, pituitary, kidney, placenta, testis, corpus luteum, adrenal glands, monocytes, prostate, bone, liver, cartilage, endothelial cells, epithelial cells
- Cell Targets
- Fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, astrocytes, oligodendrocytes, smooth muscle
- Receptors
- FGFR-1 (flg); FGFR-2 (bek, K-sam); FGFR-4 (flg-2); low affinity coreceptor heparin sulfate and heparin sulfate proteoglycans required for full activity
- Biology Area
- Angiogenesis, Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, Cytokines/Chemokines, Growth Factors
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Klagsbrun M. 1992. Semin. Cancer Biol. 3:81.
3. Goldfarb M. 1990. Cell Growth Differ. 1:439.
4. Sensenbrenner M. 1993. Prog. Neurobiol. 41:683. - Regulation
- Released after tissue injuries and during inflammatory processes
- Gene ID
- 2247 View all products for this Gene ID
- UniProt
- View information about FGF-basic on UniProt.org
Other Formats
View All FGF-basic Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-human FGF-basic | JKFb-2 | ELISA Detection,ELISPOT Detection |
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Biotin anti-human FGF-basic
