- Clone
- BVD11-37G10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Granulocyte-Colony Stimulating Factor, CSF-β, Pluripoietin, Granulocytic neutrophil colony stimulating activity (G-CSA), Macrophage-granulocyte inducer-2 (MGI-2)
- Isotype
- Rat IgG2a, κ
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 506702 | 500 µg | $328.00 | |||
Granulocyte - colony stimulating factor (G-CSF) is a potent stimulator of bone marrow cells especially those of neutrophilic granulocyte lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. G-CSF is produced primarily by monocytes and macrophages upon activation by endotoxin, TNF-alpha or IFN-gamma. The BVD11-37G10 antibody reacts with human granulocyte-colony stimulating factor (G-CSF).The BVD11-37G10 antibody can neutralize the bioactivity of natural or recombinant G-CSF.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli-expressed, recombinant human G-CSF
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection, ELISPOT Detection
- Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA or ELISPOT Detection1,2: The biotinylated BVD11-37G10 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified BVD13-3A5 antibody (Cat. No. 502102/502104) as the capture antibody.
Additional reported applications (for the relevant formats) include: Western blotting, neutralization1,2, and immunohistochemistry3,4. -
Application References
(PubMed link indicates BioLegend citation) -
- Abrams J, et al. 1995. Curr. Prot. Immunol.. John Wiley and Sons New York. Unit 6.20
- Abrams J, et al. 1992. Immunol. Rev. 127:5.
- Andersson J, et al. 1994. Immunol. 83:16.
- Sander B, et al. 1991. Immunol. Rev. 119:65.
- RRID
-
AB_2083731 (BioLegend Cat. No. 506702)
Antigen Details
- Structure
- Cytokine; 21 kD (Mammalian)
- Bioactivity
- Growth/differentiation/activating factor for neutrophils; proliferation/migration endothelial cells cells; synergistic with IL-3 growth of hematopoietic progenitors
- Cell Sources
- Macrophages, monocytes, neutrophils, fibroblasts, endothelial cells, bone marrow stromal cells
- Cell Targets
- Hematopoietic progenitors of neutrophil/granulocyte lineage, endothelial cells
- Receptors
- CD114 (G-CSFR)
- Cell Type
- Neutrophils
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Demetri G, et al. 1991. Blood 78:2791.
3. Moore M. 1991. Annu. Rev. Immunol. 9:159.
4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263. - Regulation
- Upregulated by IL-1, IL-17, TNF-α, IFN-γ, GM-CSF; downregulated by prostaglandin E2
- Gene ID
- 1441 View all products for this Gene ID
- UniProt
- View information about G-CSF on UniProt.org
Other Formats
View All G-CSF Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-human G-CSF | BVD11-37G10 | ELISA Detection,ELISPOT Detection |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Biotin anti-human G-CSF
