- Clone
- Poly5020 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-13, NC30
- Isotype
- Rabbit Polyclonal IgG
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 502001 | 50 µg | $375.00 | |||
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 lymphocytes. IL-13 shares 30% amino acid sequence homology with IL-4 and demonstrates similar biological activities. The biological activities of IL-13 include: suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of proinflammatory cytokine secretion. The poly5020 polyclonal antibody reacts with human interleukin-13 (IL-13).
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Recombinant human IL-13
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.2% BSA and 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection, ELISPOT Detection
- Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. As a detection antibody in ELISA assays, dilute 60 µl of this biotinylated antibody into 12 ml of detection buffer for one 96 well microtiter plate assay. To obtain a linear standard curve, serial dilutions of human IL-13 recombinant protein ranging from 2000 to 8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA Detection or ELISPOT Detection: The biotinylated Poly5020 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES10-5A2 (Cat. No. 501902/501910) antibody as the capture antibody.
This product may contain other non-IgG subtypes. - Additional Product Notes
-
This antibody can be used for 4 plates.
-
Application References
(PubMed link indicates BioLegend citation) -
- Bailer, R.T., et al.., 1999. J. Immunol.. 162 :7534.
- Arva, E., et al.., 1999. Scand. J. Immunol.. 49:411.
- Product Citations
-
- RRID
-
AB_2124285 (BioLegend Cat. No. 502001)
Antigen Details
- Structure
- Cytokine; 12.5 kD
- Function
- Suppression of macrophage cytotoxic activity; upregulation of IL-1RA expression; suppression of IL-1, IL-6, IL-8, IL-10, IL-12, chemokines MCP, MIP-1; differentiation of B cells, monocytes
- Bioactivity
- Suppression of macrophage cytotoxic activity; upregulation of IL-1RA expression; suppression of IL-1, IL-6, IL-8, IL-10, IL-12, chemokines MCP, MIP-1; differentiation of B cells, monocytes
- Cell Sources
- Activated T helper cells, CD8+ T cells, mast cells, NK cells
- Cell Targets
- B cells, monocytes, macrophages, basophils
- Receptors
- Moderate affinity IL-13Rα1; high affinity heterodimer IL-13Rα1/IL-4Rα also binds IL-4; both receptor complexes have γ-subunit in common with IL-2R, IL-4R, IL-7R, IL-15R ; high affinity IL-13Rα2 incapable of signaling
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hilton, D., et al. 1996. P. Natl. Acad. Sci. USA 93:497.
3. Obiri, N., et al. 1995. J. Biol. Chem. 270:8797.
4. Ochensberger, B., et al. 1996. Blood 88:3028. - Regulation
- IL-13Rα2 possible antagonist
- Gene ID
- 3596 View all products for this Gene ID
- UniProt
- View information about IL-13 on UniProt.org
Other Formats
View All IL-13 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-human IL-13 | Poly5020 | ELISA Detection,ELISPOT Detection |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Biotin anti-human IL-13
