- Clone
- Poly5189 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin IL-17A, IL-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
- Isotype
- Goat Polyclonal Ig
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 518902 | 50 µg | $375.00 | |||
IL-17A is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A, secreted by activated CD4+ Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. Recent studies demonstrated that, in mice, activated IL-17-secreting CD4+ helper T cells (Th17 cells) mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA). Human IL-17A shows 63%, 63%, and 72% amino acid sequence identity to rat IL-17A, mouse IL-17A, and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS), respectively.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Immunogen
- Recombinant human IL-17A, amino acids Ile20-Ala155 (Accession # NM_002190) was expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 1% BSA (USA origin) and 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-17A recombinant protein ranging from 1000 to 15.6 pg/ml are recommended.
- Application Notes
-
ELISA Detection: The biotinylated Poly5189 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified BL23 (Cat. No. 512702) antibody as the capture antibody and recombinant human IL-17A (Cat. No. 570509) as the standard.
- Product Citations
-
- RRID
-
AB_2561347 (BioLegend Cat. No. 518902)
Antigen Details
- Structure
- Cytokine; dimer; ~15 kD (Mammalian)
- Bioactivity
- Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
- Cell Sources
- Activated T cell subpopulation
- Cell Targets
- Fibroblasts, epithelial and endothelial cells, stromal cells
- Cross
- In sandwich ELISA, cross-reactivity with human IL-17F is negligible and no cross-reactivity with mouse IL-17A is present.
- Receptors
- IL-17R
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Hirota K, et al. 2007. J. Exp. Med. 204:41.
2. Furuzawa-Carballeda J, et al. 2007. Autoimmun. Rev. 6:169.
3. Witowski J, et al. 2007. Kidney Int. 71:514.
4. Gaffen SL, et al. 2006. Vitam. Horm. 74:255.
5. Hymowitz S, et al. 2001. EMBO J. 20:5332. - Gene ID
- 3605 View all products for this Gene ID
- UniProt
- View information about IL-17A on UniProt.org
Other Formats
View All IL-17A Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-human IL-17A | Poly5189 | ELISA Detection |
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Biotin anti-human IL-17A
