Biotin anti-mouse CD127 (IL-7Rα) Antibody

Pricing & Availability
Clone
SB/199 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Rat IgG2b, κ
SBslash199_Biotin_042909.jpg
C57BL/6 splenocytes stained with SB/199 biotin followed by Sav-PE and CD3 (145-C11) FITC
  • SBslash199_Biotin_042909.jpg
    C57BL/6 splenocytes stained with SB/199 biotin followed by Sav-PE and CD3 (145-C11) FITC
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Cat # Size Price Quantity Check Availability
121103 50 µg $106.00
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121104 500 µg $411.00
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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein, also known as IL-7 receptor α chain or IL-7Rα. It forms heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse pre-B cell line 1A9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining.

Application References

(PubMed link indicates BioLegend citation)
  1. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
  2. Kouro T, et al. 2002. Blood 100:3672.
  3. Maeda K, et al. 2005. Blood 106:879.
  4. Diao J, et al. 2004. J. Immunol. 173:1826.
Product Citations
  1. Baumgartner C, et al. 2012. J Immunol. 189:2309. PubMed
  2. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  3. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  4. Walker JA, et al. 2020. Immunity. 51(1):104-118. PubMed
  5. Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed
RRID
AB_493501 (BioLegend Cat. No. 121103)
AB_493501 (BioLegend Cat. No. 121104)

Antigen Details

Structure
Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. Proc. Natl. Acad. Sci. USA. 90:9125.
2. He YW and Malek TR. 1998. Crit Rev. Immunol. 18:503.
3. Huster K M,et al. 2004. Proc. Natl. Acad. Sci. USA. 101:5610.
4. Pillai M, et al. 2004. Leuk Lymphoma. 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.

Gene ID
16197 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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