Biotin anti-mouse CD210 (IL-10 R) Antibody

Pricing & Availability
Clone
1B1.3a (See other available formats)
Regulatory Status
RUO
Other Names
IL-10R, Interleukin-10 Receptor
Isotype
Rat IgG1, κ
1B1dot3A_Biotin_090707
C57BL/6 mouse splenocytes stained with biotinylated 1B1.3a, followed by Sav-PE
  • 1B1dot3A_Biotin_090707
    C57BL/6 mouse splenocytes stained with biotinylated 1B1.3a, followed by Sav-PE
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Cat # Size Price Quantity Check Availability
112704 500 µg $282.00
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Description

CD210 is a 90-110 kD IL-10 receptor. It is a class II cytokine receptor expressed on thymocytes, T cells, B cells, NK cells, monocytes and macrophages. Ligand binding of CD210 induces Jak1 and Tyk, resulting in STAT1 and STAT3 activation. IL-10 receptor stimulation results in the inhibition of cytokine production and the costimulation of B cell proliferation and differentiation. The only known ligand for this receptor is IL-10.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Soluble mIL-10R-FLAG-His
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of ligand binding1-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 112706) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112704) or biotinylated anti-rat IgG (Cat. No. 405402) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 112710) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. O'Farrell A, et al. 1998. EMBO J. 17:1006. (Block)
  2. Wang W, et al. 2004. FASEB J. 18:1043. (Block)
  3. Vieira PL, et al. 2004. J. Immunol. 172:5986. (Block)
  4. Iwashiro M, et al. 2001. P. Natl. Acad. Sci. USA 98:9226. (Block)
  5. Vicari AP, et al. 2002. J. Exp. Med. 196:541. (Block)
Product Citations
  1. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  2. Girard-Madoux M, et al. 2012. J Allergy Clin Immunol. 129:143. PubMed
  3. Zhou JY, et al. 2021. eLife. 10:00. PubMed
RRID
AB_313517 (BioLegend Cat. No. 112704)

Antigen Details

Structure
Class II cytokine receptor family, 90-110 kD
Distribution

Thymocytes, T and B cells, NK cells, monocytes, macrophages

Function
Inhibits cytokine production, costimulates B cell proliferation and differentiation
Ligand/Receptor
IL-10
Cell Type
B cells, Macrophages, Monocytes, NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Ho A, et al. 1993. P. Natl. Acad. Sci. USA 90:11267.
2. Tan JC, et al. 1993. J. Biol. Chem. 268:21053.
3. Niemand C, et al. 2003. J. Immunol. 170:3263.
4. Corinti S, et al. 2001. J. Immunol. 166:4312.

Gene ID
16154 View all products for this Gene ID
UniProt
View information about CD210 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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