- Clone
- A18052H (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-7, Lymphopoietin-1 (LP-1), Pre-B cell growth factor, Thymocyte growh factor
- Isotype
- Rat IgG2a, κ
-
Direct ELISA showing biotin anti-mouse IL-7 (clone A18052H) bound to plate-immobilized recombinant mouse IL-7 protein (Cat Nos. 577802, 577804, 577806, and 577808). The wells were incubated with serially diluted primary biotin antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Avidin (Cat. No. 405103). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 630253 | 50 µg | $495.00 | |||
Mouse IL-7 was initially described as a pre-B-cell grow factor expressed in bone marrow stromal cells. IL-7 is essential for normal murine B cell development, and plays a key role in regulating the homeostasis and function of the T-cells. The binding of IL-7 to its receptor induces dimerization of IL-7Ra and the common gamma chain (gc) which leads to activation of receptor associated tyrosine Janus kinases JAK1 (IL-7R) and JAK3 (γc). The activated JAK proteins in turn phosphorylate specific residues on the IL-7R creating docking sites for signaling molecules such as STAT5 and to a lesser extent STAT1 and STAT3. In adult mice, IL-7/IL-7R signaling up regulates expression of early B cell factor (EBF), and EBF in turn regulates expression of B cell-specific genes required for the transition from lymphoid progenitor to pro-B cells. Besides, IL-7 plays a role in the development of secondary lymphoid tissues. IL-7 is necessary to specify CD8 lineage differentiation during CD4/CD8 cell fate choice in the thymus by inducing expression of the transcription factor Runx3. IL-7 induces antiapoptotic factors Bcl2 and Bcl-xL and inhibiting pro-apoptotic factors such as Bad and Bax. In this fashion, IL-7 induces cell activation, survival, and proliferation of T lymphocytes. In addition, IL-7 controls T-cell size and metabolism through the activation of PI3 kinase-dependent pathways and regulation of glucose metabolism. IL-7 also controls T cell–dendritic cell interactions that are essential for both T-cell homeostasis and activation in vivo. CD4 T cell lymphopenia increases the expression of circulating IL-7, and TGFb induces IL-7 downregulation.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse Recombinant IL-7 Protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
Direct ELISA - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
- Additional Product Notes
-
This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A18052H) could function as a detection antibody when paired with purified anti-mouse IL-7 antibody (Cat. No. 630452) (clone A18052C) as the capture antibody and using Recombinant Mouse IL-7 protein (Cat Nos. 577802, 577804, 577806, and 577808) as the assay standard.
- RRID
-
AB_3097337 (BioLegend Cat. No. 630253)
Antigen Details
- Structure
- Cytokine
- Distribution
-
Thymic and bone morrow stromal cells, keratinocytes, dendritic cells, endothelial cells, intestinal epithelial, and epithelial goblet cells
- Function
- IL-7 is essential for B cell production
- Interaction
- Progenitor B and T cells, CD4-CD8- thymocytes, naïve and memory CD4 and CD8 T cells, and megakaryocytes
- Ligand/Receptor
- Heterodimer IL-7Rα (CD127); γ-subunit (CD132) which shares component with IL-2, IL-4, IL-13, IL-15, and IL-21 receptors
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Cancer Biomarkers, Neuroinflammation, Neuroscience, Stem Cells
- Antigen References
-
- Yokota T, et al. 1986. P Natl Acad Sci USA. 83:5894.
- Meier D, et al. 2007. Immunity 26:643-54.
- Parish JK, et al. 2009. J Immunol. 182:4255-66.
- Saini M, et al. 2009. Blood. 113:5793-800.
- Park JH, et al. 2010. Nat Immunol. 11:257-64.
- Li WQ, et al. 2010. Mol Cell Biol. 30:590-600.
- Gene ID
- 16196 View all products for this Gene ID
- UniProt
- View information about IL-7 on UniProt.org
Other Formats
View All IL-7 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse IL-7 | A18052H | Direct ELISA |
| Biotin anti-mouse IL-7 | A18052H | Direct ELISA |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-mouse IL-7
Direct ELISA showing purified anti-mouse IL-7 (clone A18052H... -
Biotin anti-mouse IL-7
Direct ELISA showing biotin anti-mouse IL-7 (clone A18052H) ...