Biotin anti-mouse MCP-1 Antibody

Pricing & Availability
Clone
4E2/MCP (See other available formats)
Regulatory Status
RUO
Other Names
Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2
Isotype
Hamster IgG
Cat # Size Price Quantity Check Availability
506002 500 µg $319.00
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Description

Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 4E2/MCP antibody reacts with mouse MCP-1. This antibody does not react with human MCP-1 and related mouse beta chemokines, TCA3 and MIP-1beta. The 4E2/MCP antibody can not neutralize the bioactivity of natural or recombinant MCP-1.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Hamster
Immunogen
CHO-expressed, recombinant mouse MCP-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection, ELISPOT Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture: The purified 4E2/MCP antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 2H5 antibody (Cat. No. 505908) as the detecting antibody for the detection of mouse MCP-1. The LEAF™ purified antibody is suggested for ELISPOT capture.
Additional reported applications (for the relevant formats) include: Western blotting1. The 4E2/MCP is a non-neutralizing antibody1.

Application References

(PubMed link indicates BioLegend citation)
  1. Luo Y, et al. 1994. J. Immunol. 153:3708.
  2. Luo Y, et al. 1999 J. Immunol. 163:3985.
Product Citations
  1. Yang D, et al. 2013. Cereb Cortex. 23:1218. PubMed
  2. Feig J, et al. 2011. PLoS One. 6:e28534. PubMed
RRID
AB_2281903 (BioLegend Cat. No. 506002)

Antigen Details

Structure
CC chemokine; dimer; 13.8 kD (Mammalian)
Bioactivity
Chemoattractant; regulates adhesion molecule expression, cytokine production in monocytes; proliferation/activation of CC-chemokine activated killer cells
Cell Sources
Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells
Cell Targets
Monocytes, basophils, T cells, NK cells, immature dendritic cells
Receptors
CCR2; CCR5; CCR10; D6; possibly L-CCR
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bischoff S, et al. 1992. J. Exp. Med. 175:1271.
3. Charo I, et al. 1994. P. Natl. Acad. Sci. USA 91:2752.
4. Jiang Y, et al. 1992. J. Immunol. 148:2423.

Regulation
Upregulated by IL-1, TNF-a, PDGF, TGF-ß
Gene ID
20296 View all products for this Gene ID
UniProt
View information about MCP-1 on UniProt.org

Other Formats

View All MCP-1 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse MCP-1 4E2/MCP ELISA Detection,ELISPOT Detection
Purified anti-mouse MCP-1 4E2/MCP ELISA Capture,WB
Go To Top Version: 3    Revision Date: 08/29/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Biotin anti-mouse MCP-1

    MCP-1_ELISA_071805
  • Purified anti-mouse MCP-1

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