Biotin anti-mouse / rat IL-1β Antibody

Product Details

Verified Reactivity

Mouse, Rat

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

E. coli -expressed, recombinant mouse IL-1β

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay.

Application Notes

ELISA or ELISPOT Capture³: The purified B122 antibody is useful as the capture antibody in a mouse IL-1ß sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5158 antibody (Cat. No. 515801) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.

Certain formats of clone B122 have been reported for in vitro neutralization of IL-1ß bioactivity¹, in vivo cytokine depletion⁵, immunohistochemical staining of frozen sections⁶, Western blotting⁷, and the study of endotoxin shock⁸.

Application References

(PubMed link indicates BioLegend citation)

1. Hogquist K, et al. 1991. J. Immunol. 146:1534.
2. Williams RO, et al. 2000. J. Immunol. 165:7240. (Neut)
3. Brewer J, et al. 1998. J. Immunol. 161:4000. (ELISA and ELISPOT Capture)
4. Rogers HW, et al. 1992. Proc. Natl. Acad.Sci. USA 89:1011.
5. Cantwell AM, et al. 2010. BMC Immunol. 11:29. (Depletion)
6. Dube PH, et al. 2001. Proc. Natl. Acad. Sci. USA 98:10880. (IHC)
7. Babelova A, et al. 2009. J. Biol. Chem. 284:24035. (WB)
8. Lu JY, et al. 2006. Genes Dev. 20:3174. (Neut) PubMed

Product Citations

1. Bulek K, et al. 2020. J Clin Invest. 130:4218. PubMed
2. Allen KD, et al. 2020. Connect Tissue Res. 61:82. PubMed

RRID

AB_2295906 (BioLegend Cat. No. 503505)

Antigen Details

Structure

Cytokine; 17.5 kD (Mammalian)

Bioactivity

Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages

Cell Sources

Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma,

Cell Targets

B cells, T cells, monocytes

Receptors

Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)

Biology Area

Immunology, Innate Immunity

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.

Regulation

Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α₂-macroglobulin

Gene ID

16176 View all products for this Gene ID

UniProt

View information about IL-1beta on UniProt.org

Related Protocols

• Sandwich ELISA Protocol

Other Formats