- Clone
- W3/13 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, W3/13
- Isotype
- Mouse IgG1, κ
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LOU rat splenocytes stained with W3/13 biotin, followed by Sav-PE
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 202803 | 100 µg | $153.00 | |||
CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and W3/13, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.
Product Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Rat thymocyte membrane glycoproteins
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections1 and paraffin-embedded sections4.
-
Application References
(PubMed link indicates BioLegend citation) -
- Barclay AN. 1981. Immunology 42:593.
- Brown WRA, et al. 1981. Nature 289:456.
- Forbes RC, et al. 1983. Transplantation 36:681.
- Jung S, et al. 1994. Immunology 83:545.
- Product Citations
-
- RRID
-
AB_528808 (BioLegend Cat. No. 202803)
Antigen Details
- Structure
- Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 95-130kD
- Distribution
-
Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, activated B cells, and hemotopoietic progenitor cells.
- Function
- Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors
- Ligand/Receptor
- CD54, Siglec-1
- Cell Type
- B cells, Granulocytes, Hematopoietic stem and progenitors, Monocytes, NK cells, Plasma cells, Platelets, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Neil Barclay A, et al. 1997. The Leucocyte Antigen Facts Book:Second Edition. Page 238
- Killeen A, et al. 1987. EMBO J. 6(13):4029.
- Thurman EC, em>et al. 1998. Intl. Immunol. 10:691.
- Onami TM, et al. 2002. J. Immunol. 168:6022.
- Tong J, et al. 2004. J. Exp. Med. 199:1277.
- Jones AT, et al. 1994. J. Immunol. 153:3426.
- Gene ID
- 20737 View all products for this Gene ID
- UniProt
- View information about CD43 on UniProt.org
Other Formats
View All CD43 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-rat CD43 | W3/13 | FC |
| Alexa Fluor® 647 anti-rat CD43 | W3/13 | FC |
| PE anti-rat CD43 | W3/13 | FC |
| PE/Cyanine7 anti-rat CD43 | W3/13 | FC |
| PerCP/Cyanine5.5 anti-rat CD43 | W3/13 | FC |
| APC anti-rat CD43 | W3/13 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-rat CD43
LOU rat splenocytes stained with W3/13 biotin, followed by S... -
Alexa Fluor® 647 anti-rat CD43
LOU rat splenocytes stained with W3/13 Alexa Fluor® 647 -
PE anti-rat CD43
LOU rat splenocytes stained with W3/13 PE -
PE/Cyanine7 anti-rat CD43
LOU rat splenocytes were stained with CD43 (clone W3/13) PE/... -
PerCP/Cyanine5.5 anti-rat CD43
LOU rat splenocytes were stained with CD43 (clone W3/13) Per... -
APC anti-rat CD43
LOU rat splenocytes were stained with CD43 (clone W3/13) APC...