Biotin anti-rat CD86 Antibody

Pricing & Availability
Clone
24F (See other available formats)
Regulatory Status
RUO
Other Names
B7-2, B70
Isotype
Mouse IgG1, κ
24F_Biotin_082109
LPS-stimulated (3 days) LOU rat splenocytes stained with biotinylated 24F, followed by SAV-PE
  • 24F_Biotin_082109
    LPS-stimulated (3 days) LOU rat splenocytes stained with biotinylated 24F, followed by SAV-PE
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Cat # Size Price Quantity Check Availability
200303 50 µg $101.00
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Description

CD86 is an 80 kD member of the immunoglobulin superfamily also known as B70 or B7-2. It is expressed on antigen presenting cells and activated T and B cells. CD86 is a ligand for CD28 and CD152 (CTLA-4). It is one of the accessory molecules that plays an important role in T cell-APC cell costimulatory interactions. The 24F antibody can block the co-stimulatory activity of CD86.

Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
HTLV-1-transformed Lewis-S1 rat T cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of costimulation of T cells1-3, and immunohistochemical staining of acetone-fixed frozen sections3. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 200310).

Application References

(PubMed link indicates BioLegend citation)
  1. Maeda K, et al. 1997. Int. Immunol. 9:993. (IP, Block)
  2. Bluestone JA. 1995. Immunity 2:555. (IP, Block)
  3. Damoiseaux JG, et al. 1998. J. Leuk. Biol. 64:803. (IP, Block, IHC)
  4. Donius LR, et al. 2014. Immunobiology. 219:440. PubMed
Product Citations
  1. Bove GM et al. 2017. PloS one. 12(6):e0178407 . PubMed
RRID
AB_313852 (BioLegend Cat. No. 200303)

Antigen Details

Structure
Ig superfamily, 80 kD
Distribution

Antigen presenting cells, activated B cells and T cells

Function
T and B cell costimulation
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
B cells, T cells, Antigen-presenting cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Ann. Rev. Immunol. 20:29.

Gene ID
56822 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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