Biotin anti-rat IgM Antibody

Pricing & Availability
Clone
MRM-47 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Mouse IgG
MRM-47_Biotin_IgM_Antibody_012022
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of GL7 (clone GL7) purified, CD3 (clone 17A2) Alexa Fluor® 647 (green) and B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) overnight at 4°C, followed by 2.5 µg/ml of anti-rat IgM (clone MRM-47) Biotin for 2 hours, followed by 2.5 µg/ml of Streptavidin Alexa Fluor® 594 (red) for 2 hours at room temperature. The image was captured by 10X objective.
  • MRM-47_Biotin_IgM_Antibody_012022
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of GL7 (clone GL7) purified, CD3 (clone 17A2) Alexa Fluor® 647 (green) and B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) overnight at 4°C, followed by 2.5 µg/ml of anti-rat IgM (clone MRM-47) Biotin for 2 hours, followed by 2.5 µg/ml of Streptavidin Alexa Fluor® 594 (red) for 2 hours at room temperature. The image was captured by 10X objective.
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Cat # Size Price Quantity Check Availability
408903 100 µg $183.00
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Description

The MRM-47 monoclonal antibody reacts with rat immunoglobulin M (IgM) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRM-47 antibody can be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.

Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mixed rat Igs
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume.  For immunohistochemistry, a concentration range of 2.5 - 5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Li K, et al. 2022. Cancer Cell. 40:1374. PubMed
  2. Liu YY, et al. 2020. Chin Med J (Engl). 133:1688. PubMed
  3. Muppidi J, et al. 2011. J Exp Med. 208:1941. PubMed
  4. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
RRID
AB_10571208 (BioLegend Cat. No. 408903)
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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