Brilliant Violet 421™ anti-human CD11b Antibody

Pricing & Availability
Clone
LM2 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM
Isotype
Mouse IgG1, κ
LM2_BV421_CD11b_Antibody_072318
Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with CD11b (clone LM2) Brilliant Violet 421™ (left) or Mouse IgG1, κ Brilliant Violet 421™ isotype control (right).
  • LM2_BV421_CD11b_Antibody_072318
    Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with CD11b (clone LM2) Brilliant Violet 421™ (left) or Mouse IgG1, κ Brilliant Violet 421™ isotype control (right).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability
393113 25 tests $187.00
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393114 100 tests $396.00
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Description

CD11b is a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, and factor X.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
β2 integrins from granulocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

LM2 does not block clone ICRF44 indicating a unique epitope.

Product Citations
  1. Li K, et al. 2022. Cancer Cell. 40:1374. PubMed
  2. Zhang TR, et al. 2023. Pharmaceuticals (Basel). 16:. PubMed
RRID
AB_2750258 (BioLegend Cat. No. 393113)
AB_2750258 (BioLegend Cat. No. 393114)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 165-170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subset of T cells, subset of B cells

Function
Adhesion, phagocytosis, chemotaxis, neutrophil activation
Ligand/Receptor
ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
Cell Type
Granulocytes, Monocytes, Macrophages, Dendritic cells, NK cells, T cells, B cells, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Stewart M, et al. 1995. Curr Opin Cell Biol. 7:690.
  2. Miller LJ, et al. 1986. J Immunol. 137(9):2891.
Gene ID
3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org
Go To Top Version: 1    Revision Date: 07/23/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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