Brilliant Violet 421™ anti-human CD195 (CCR5) Antibody

Pricing & Availability
Clone
J418F1 (See other available formats)
Regulatory Status
RUO
Other Names
C-C chemokine receptor type 5 (CCR5), HIV-1 fusion co-receptor
Isotype
Rat IgG2b, κ
J418F1_BV421_CD195_Antibody_FC_051414
Human peripheral blood lymphocytes were stained with CD195 (clone J418F1) Brilliant Violet 421™ (filled histogram) or rat IgG2b, κ Brilliant Violet 421™ isotype control (open histogram).
  • J418F1_BV421_CD195_Antibody_FC_051414
    Human peripheral blood lymphocytes were stained with CD195 (clone J418F1) Brilliant Violet 421™ (filled histogram) or rat IgG2b, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability
359117 25 tests $193.00
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359118 100 tests $424.00
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Description

CD195, also known as CCR5, is a 45 kD G protein-coupled seven transmembrane CC-chemokine receptor. It binds to MIP-1α, MIP-1β, and RANTES and is expressed on a subset of T cells and monocytes. CCR5 mediates an intracellular signal thought to induce cell differentiation and proliferation. CCR5 has also been shown to act as a co-receptor for R5 HIV-1 cell entry; modification of CCR5 by sulfation contributes to the efficiency of HIV-1 entry. Studies have shown CCR5 to play a role in a variety of other human diseases, ranging from infectious and inflammatory diseases to cancer.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CCR5 transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Barber-Axthelm IM, et al. 2023. iScience. 26:106269. PubMed
  2. Koutsakos M, et al. 2023. Immunity. 56:879. PubMed
  3. Wragg KM, et al. 2020. Cell Reports. 31(11):107773. PubMed
  4. Neidleman J, et al. 2020. Elife. 9:00. PubMed
  5. Pedersen JG, et al. 2021. Front Microbiol. 12:763030. PubMed
  6. Bellini N, et al. 2022. iScience. 25:105234. PubMed
  7. Parsons MS, et al. 2021. EBioMedicine. 70:103518. PubMed
  8. Shan L, et al. 2017. Immunity. 47:766. PubMed
  9. Cortés–Rubio CN, et al. 2019. Clin Epigenetics. 11:134. PubMed
RRID
AB_2563576 (BioLegend Cat. No. 359117)
AB_2563576 (BioLegend Cat. No. 359118)

Antigen Details

Structure
G-coupled receptor family 1, membrane protein, 45 kD
Distribution

Subset of T cells, monocytes

Function
Binds C-C chemokines and transduces an intracellular signal thought to result in proliferation and differentiation, acts as a co-receptor with CD4 for HIV-1
Ligand/Receptor
MIP-1α, MIP-1β, RANTES
Cell Type
Monocytes, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Samson M, et al. 1996. Biochemistry 35:3362.
2. Raport CJ, et al. 1996. J. Biol. Chem. 271:17161.
3. Combadiere C, et al. 1996. J. Leukoc. Biol. 60:147.
4. Deng H, et al. 1996. Nature 381:661.
5. Lai J, et al. 2003. CVI. 10:1123.
6. Mañes S, et al. 2003. J. Exp. Med. 198:1381.
7. Vaday GG, et al. 2006. Prostate 66:124.

Gene ID
1234 View all products for this Gene ID
UniProt
View information about CD195 on UniProt.org
Go To Top Version: 1    Revision Date: 06/17/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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