Brilliant Violet 421™ anti-human CD227 (MUC-1) Antibody

Pricing & Availability
Clone
16A (See other available formats)
Regulatory Status
RUO
Other Names
Mucin-1, MUC-1, Episialin, HMFG antigen, MAM6
Isotype
Mouse IgG1, λ
16A_BV421_CD227_Antibody_071123
PHA-stimulated (3-days) human peripheral blood mononuclear cells were stained with anti-human CD227 (MUC-1) (clone 16A) Brilliant Violet 421™ (left), or mouse IgG1 Brilliant Violet 421™ isotype control (right). Cells were co-stained with anti-human CD3 FITC.
  • 16A_BV421_CD227_Antibody_071123
    PHA-stimulated (3-days) human peripheral blood mononuclear cells were stained with anti-human CD227 (MUC-1) (clone 16A) Brilliant Violet 421™ (left), or mouse IgG1 Brilliant Violet 421™ isotype control (right). Cells were co-stained with anti-human CD3 FITC.
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355615 25 tests $193.00
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355616 100 tests $407.00
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Description

Mucin-1 (MUC-1), cell surface associated or polymorphic epithelia mucin, is a 500-1000 kD proteoglycan expressed by activated T cells, mucosal epithelial cells, and aberrantly expressed on most breast cancers.  In normal cells, CD227 is heavily glycosylated, whereas in cancerous cells, the glycosylation is incomplete and premature sialation is also observed.  The protein is anchored to the apical surface of the epithelial cell and functions as a lubricant to keep the cell hydrated and to protect against pathogens.  It can also function as a signaling molecule by forming a MUC-1/SOS1/GrB2 complex.  MUC-1 can interact with cancer antigens such as Her2/neu.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Jurkat cells transfected with MUC1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

This clone shows stronger binding to the glycosylated compared to the non-glycosylated peptide1.

Application References

(PubMed link indicates BioLegend citation)
  1. Song W, et al. 2012. Int. J Oncol. 41:1977. PubMed
RRID
AB_3083339 (BioLegend Cat. No. 355615)
AB_3083339 (BioLegend Cat. No. 355616)

Antigen Details

Structure
500-1000 kD proteoglycan (glycosylated form)
Distribution

Activated T cells, mucosal epithelial cells, aberrantly expressed in most breast cancers

Interaction
Complexes with SOS1 and Grb2 in RAS signaling
Ligand/Receptor
ICAM-1 and Her2/neu (ErbB2)
Cell Type
Dendritic cells, Epithelial cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Gendler SJ. 2001. J. Mammary Gland Biol. Neoplasia. 6:339.
2. Agrawal B, et al. 1998. Cancer Res. 58:4079.
3. Rahn JJ, et al. 2004. J. Biol. Chem. 279:29386.

Gene ID
4582 View all products for this Gene ID
UniProt
View information about CD227 on UniProt.org
Go To Top Version: 1    Revision Date: 07/11/2023

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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