- Clone
- MHN4-2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Neurogenic locus notch homolog protein 4
- Isotype
- Mouse IgG1, κ
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Human acute lymphoblastic leukemia cells HPB-ALL were stained with anti-human Notch 4 (clone MHN4-2) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 349007 | 25 tests | $220.00 | |||
| 349008 | 100 tests | $440.00 | |||
Neurogenic locus notch homolog protein 4 (Notch 4) is a transmembrane heterodimer in which the extracellular domain contains 29 EGF repeats and 3 LNR repeats. The intracellular domain contains 5 ANK repeats, 1 PEST motif, and 1 RAM23 domain. Notch 4 controls many developmental processes in the cell such as differentiation, proliferation, and apoptosis. In addition, it also plays a role in vascular, renal, and hepatic development. Notch 4 is expressed by a variety of tissues such as heart, lung, placenta, liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow, and fetal liver. The Notch 4 ligands are Jagged1, Jagged2, Delta1, Delta3 and Delta4. After ligand binding, Notch 4 is cleaved by the TNF-α converting enzyme (TACE). The extracellular domain remains on the cell surface while the intracellular domain is released and translocated to the nucleus where it binds to CBF-1, leading to the expression of genes of the Hairy/Enhancer of Split (HES) and HES-related repressor protein (HERP) families.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Notch 4-transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- RRID
-
AB_3662247 (BioLegend Cat. No. 349007)
AB_3662247 (BioLegend Cat. No. 349008)
Antigen Details
- Structure
- Transmembrane protein. Extracellular domain forms a heterodimer and contains 29 epidermal growth factor-like repeats and 3 Lin/Notch Glp repeats. The intracellular domain contains 5 CDC10/Ankryn repeats, 1 proline, glutamate, serine, threonine-rich motif,
- Distribution
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Heart, lung, placenta, liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow, and fetal liver.
- Function
- Controls many cell developmental processes such as differentiation, proliferation and apoptosis. Plays a role in vascular, renal, and hepatic development.
- Interaction
- MAML1, MAML2 and MAML3, CBF-1, Smad3, and Smad4
- Ligand/Receptor
- Jagged1, Jagged2, Delta1, Delta3, and Delta4
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Transcription Factors
- Antigen References
-
1. Katoh M and Katoh M. 2007. Int. J. Oncol. 30:247.
2. Kerbel RS. 2008. N. Engl. J. Med. 358:2039.
3. Chen VC, et al. 2008. Nat. Biotechnol. 26:1169.
4. Emuss V. 2009. PLoS Pathog. 5:e1000616.
5. Harrison H, et al. 2010. Cancer Res. 70:709. - Gene ID
- 929 View all products for this Gene ID
- UniProt
- View information about Notch 4 on UniProt.org
Other Formats
View All Notch 4 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human Notch 4 | MHN4-2 | FC |
| PE anti-human Notch 4 | MHN4-2 | FC |
| APC anti-human Notch 4 | MHN4-2 | FC |
| Brilliant Violet 421™ anti-human Notch 4 | MHN4-2 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human Notch 4
Human acute lymphoblastic leukemia cells HPB-ALL stained wit... -
PE anti-human Notch 4
Human acute lymphoblastic leukemia cells HPB-ALL stained wit... -
APC anti-human Notch 4
Human acute lymphoblastic leukemia cells HPB-ALL stained wit... -
Brilliant Violet 421™ anti-human Notch 4
Human acute lymphoblastic leukemia cells HPB-ALL were staine...