Brilliant Violet 421™ anti-mouse CD34 Antibody

Pricing & Availability
Clone
SA376A4 (See other available formats)
Regulatory Status
RUO
Other Names
My10, gp105-120, mucosialin
Isotype
Rat IgG2a, λ
SA376A4_BV421_CD34_Antibody_1_012017
C57BL/6 mouse bone marrow cells were stained with anti-mouse Lineage Cocktail PE and CD34 (clone SA376A4) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom). Data shown was gated on the total cell population.
  • SA376A4_BV421_CD34_Antibody_1_012017
    C57BL/6 mouse bone marrow cells were stained with anti-mouse Lineage Cocktail PE and CD34 (clone SA376A4) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom). Data shown was gated on the total cell population.
  • SA376A4_BV421_CD34_Antibody_2_012017
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability
152207 25 µg $198.00
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152208 100 µg $413.00
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Description

CD34, also known as gp105-120, is a highly glycosylated type I monomeric sialomucin-like glycophophoprotein with an approximate molecular weight of 105-120 kD, with two generated by alternative splicing. CD34 is expressed on hematopoietic progenitors, endothelial cells, brain, and testis. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands, and mediates the attachment of stem cells to the extracellular matrix or stromal cells. CD34 is phosphorylated on serine residues by PKC.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD34 transfected cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

This antibody (clone SA376A4) is useful for staining mouse CD34+ hematopoietic stem cell but it does not stain NIH-3T3 cells and the staining of Bend.3 cells is dimmer compared to other mouse CD34 antibodies, probably due to this antibody recognizes a different epitope than the other monoclonal antibodies.

Product Citations
  1. Vanneste D, et al. 2023. Nat Immunol. 24:827. PubMed
  2. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  3. Pioli PD, et al. 2020. Immunity. 51(2):351-366. PubMed
  4. Thinard R, et al. 2022. Pharmaceutics. 14:. PubMed
  5. Montecino-Rodriguez E, et al. 2020. STAR Protoc. 1:100159. PubMed
  6. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  7. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  8. Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
  9. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
RRID
AB_2650765 (BioLegend Cat. No. 152207)
AB_2650765 (BioLegend Cat. No. 152208)

Antigen Details

Structure
Type I monomeric glycophophoprotein with an approximate molecular weight of 105-120 kD.
Distribution

Hematopoietic progenitors, endothelial cells, brain and testis tissues.

Function
Cell adhesion and homing.
Interaction
PKC.
Ligand/Receptor
L-selectin and E-selectin.
Cell Type
Endothelial cells, Hematopoietic stem and progenitors
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Garlanda C, et al. 1997. Eur J Cell Biol. 73:368-77.
2. Brown J, et al. 1991. Int Immunol. 3:175.
3. Suda J, et al. 1992. Blood. 79:2288-95.
4. Baumhueter S, et al. 1994. Blood. 84:2554.

Gene ID
12490 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org
Go To Top Version: 1    Revision Date: 01/23/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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