Brilliant Violet 421™ anti-mouse CD64 (FcγRI) Antibody

Pricing & Availability
Clone
X54-5/7.1 (See other available formats)
Regulatory Status
RUO
Other Names
FcRI
Isotype
Mouse IgG1, κ
X54-5.7.1_BV421_CD64_Antibody_FC_1_102813.jpg
C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD64 (clone X54-5/7.1) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom). Data shown was gated on myeloid cell population.
  • X54-5.7.1_BV421_CD64_Antibody_FC_1_102813.jpg
    C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD64 (clone X54-5/7.1) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom). Data shown was gated on myeloid cell population.
  • X54-5.7.1_BV421_CD64_Antibody_FC_2_102813.jpg
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability
139309 50 µg $292.00
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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.75 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.

Application References

(PubMed link indicates BioLegend citation)
  1. Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
  2. Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
  3. Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
  4. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
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  18. Kovács B, et al. 2020. Front Immunol. 2.508333333. PubMed
  19. Zhang S, et al. 2019. Cell Metab. 29:443. PubMed
  20. Ku MW, et al. 2020. Cell Host Microbe. 29(2):236-249.e6. PubMed
  21. Schippers A, et al. 2015. Mucosal Immunol. 101038/mi201582. PubMed
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  23. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
  24. Lim HY et al. 2018. Immunity. 49(2):326-341 . PubMed
  25. Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
  26. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  27. Branchett WJ, et al. 2020. Wellcome Open Research. 0.278472222. PubMed
  28. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  29. Riding AM, et al. 2022. iScience. 25:104660. PubMed
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  32. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  33. Martinez-Turtos A, et al. 2022. Oncoimmunology. 11:2116844. PubMed
  34. Wang Y, et al. 2020. eLife. 9:e57438.. PubMed
  35. Wu M, et al. 2020. Mucosal Immunol. 13:47. PubMed
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RRID
AB_2562694 (BioLegend Cat. No. 139309)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, mast cells, dendritic cells

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG
Cell Type
Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14129 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org
Go To Top Version: 2    Revision Date: 12/14/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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