Brilliant Violet 421™ anti-mouse/human IL-5 Antibody

Product Details

Verified Reactivity

Mouse, Human

Reported Reactivity

Guinea Pig

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Partially-purified T cell clone supernatant

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

ELISA^1,3,4 or ELISPOT^2,6 Capture: The purified TRFK5 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated TRFK4 antibody (Cat. No. 504402) as the detection antibody for mouse IL-5, or used in conjunction with biotinylated JES1-5A10 antibody (Cat. No. 501002) as the detection antibody for human IL-5. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.

Flow Cytometry: The fluorochrome-labeled TRFK51 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-5-producing cells within mixed cell populations.

Neutralization^1,9: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-5 bioactivity (Cat. Nos. 504313-504318).

Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections⁸ and paraformaldehyde-fixed, saponin-treated frozen tissue sections⁷, and immunocytochemistry.

Note: For testing human IL-5 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430401 and 430404) are specially developed and recommended. For testing mouse IL-5 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. Nos. 431201 and 431206) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)

1. Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
2. Klinman, D., et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19.
3. Swain, S., et al. 1990. J. Immunol. 145:3796.
4. Abrams, J., et al. 1992. Immunol. Rev. 127:5.
5. Assenmacher, M., et al. 1994. Eur. J. Immunol. 24:1097.
6. Karulin, A., et al. 2000. J. Immunol. 164:1862.
7. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
8. Fan, W. Y., et al. 2001. Exp. Biol. Med. 226:1045.
9. Sitkauskiene, B., et al. 2005. Respir Res. 6:33.

Product Citations

1. Pohlmeier L, et al. 2021. Allergy. 76:2030. PubMed
2. Liao W, et al. 2023. Br J Pharmacol. 180:1056. PubMed
3. Fu Z, et al. 2019. Cell Rep. 28:159. PubMed
4. Golebski K, et al. 2019. Nat Commun. 10:2162. PubMed
5. Kaur K, et al. 2021. Cell Rep. 37:110178. PubMed
6. Wu G, et al. 2020. Br J Pharmacol. 177:5063. PubMed
7. Chinthrajah S, et al. 2019. JCI Insight. 4:e131347. PubMed
8. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
9. Holly Bachus et al. 2018. Immunity. 50(1):225-240 . PubMed
10. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
11. Surace L, et al. 2021. Nat Immunol. 22:1367. PubMed
12. Fu Z, et al. 2021. Nat Commun. 12:4462. PubMed
13. Bosnjak B, et al. 2021. Front Immunol. 12:772240. PubMed
14. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
15. Morimoto Y, et al. 2018. Immunity. 49:134. PubMed
16. Kohn EM, et al. 2022. J Immunol. 208:1417. PubMed
17. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
18. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
19. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
20. Lambert EE, et al. 2020. Vaccines (Basel). 8:. PubMed

RRID

AB_2563161 (BioLegend Cat. No. 504311)

Antigen Details

Structure

Cytokine; dimer; 40-50 kD (Mammalian)

Bioactivity

Growth/differentiation of B cells, eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes

Cell Sources

Mast cells, T cells, eosinophils

Cell Targets

Eosinophils, B cells

Receptors

Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR

Biology Area

Immunology

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Takatsu, K., et al. 1988. Immunol. Rev. 102:107.
3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299.
4. Takatsu, K. 1991. Microbiol. Immunol. 35:593.

Gene ID

16191 View all products for this Gene ID 3567 View all products for this Gene ID

UniProt

View information about IL-5 on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Other Formats