Brilliant Violet 421™ anti-mouse IL-2 Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E. coli-expressed, recombinant mouse IL-2

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.

Concentration

µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

ELISA Detection^1-3 or ELISPOT Detection^4-6: The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. Nos. 503701 & 503702) as capture antibody and recombinant mouse IL-2 (Cat. No. 575409) as the standard.
Flow Cytometry^8-10: The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations.
Neutralization^1,7: The Ultra-LEAF™ purified antibody (Endotoxin in vivo and in vitro (Cat. No. 503845-503850)) is recommended for neutralization.
Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections², in vivo capture⁷, and immunocytochemistry.
Note: For testing mouse IL-2 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. No. 431001 & 431004) are specially developed and recommended.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library. 

Application References

(PubMed link indicates BioLegend citation)

1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
2. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
3. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20.
4. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19.
5. Mo X, et al. 1995. J. Virol. 69:1288.
6. Karulin A, et al. 2000. J. Immunol. 164:1862.
7. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28.
8. Ko SY, et al. 2005. J. Immunol. 175:3309. PubMed
9. Kang SS and Allen PM. 2005. J. Immunol. 174:5382.
10. Lawson BR, et al. 2007. J. Immunol. 178:5366.

Product Citations

1. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
2. Ai L, et al. 2023. Cell Discov. 9:9. PubMed
3. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
4. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
5. Haratani K, et al. 2019. J Clin Invest. 130:374. PubMed
6. Elong Ngono A, et al. 2020. Cell Reports. 1.330555556. PubMed
7. Ma J, et al. 2020. Adv Sci (Weinh). 7:2000609. PubMed
8. Kinder JM, et al. 2020. Cell Reports. 31(12):107784.. PubMed
9. Chakrabarti J, et al. 2018. Oncotarget. 9:37439. PubMed
10. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
11. Pishesha N, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
12. Bullard BL, et al. 2022. NPJ Vaccines. 7:65. PubMed
13. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
14. Morris AB, et al. 2020. Immunity. 52(1):136-150.e6.. PubMed
15. Dudeck J, et al. 2019. J Allergy Clin Immunol. 143:1849. PubMed
16. Marshall N, et al. 2015. Toxicol Sci. 147: 127-139. PubMed
17. Karanika S, et al. 2022. Front Immunol. 13:972266. PubMed
18. Wen J, et al. 2020. Cell Rep. 31:107566. PubMed

RRID

AB_2650897 (BioLegend Cat. No. 503825)
AB_2650897 (BioLegend Cat. No. 503826)

Antigen Details

Structure

Cytokine; 15-30 kD (Mammalian)

Bioactivity

Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance

Cell Sources

T cells

Cell Targets

T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes

Receptors

High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R

Cell Type

Tregs

Biology Area

Immunology

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.

Regulation

Upregulated by NFAT; downregulated by TCF-8, CIF (colostrum inhibitory factor)

Gene ID

16183 View all products for this Gene ID

UniProt

View information about IL-2 on UniProt.org

Related Protocols

• Surface and Intracellular Cytokine Staining for Flow Cytometry - Video
• Intracellular Flow Cytometry Staining Protocol

Other Formats