Brilliant Violet 421™ anti-mouse LAP (TGF-β1) Antibody

Pricing & Availability
Clone
TW7-16B4 (See other available formats)
Regulatory Status
RUO
Other Names
Latency Associated Peptide (LAP), Transforming growth factor-beta 1 (TGF-b1), TGFB1, DPD1, TGF-beta1
Isotype
Mouse IgG1, κ
TW7-16B4_BV421_LAP_Antibody_FC_1_091912
C57BL/6 mouse splenocytes were stimulated with CD3, CD28, and recombinant mouse IL-2 for 48 hours, then surface stained with CD4 FITC and LAP (TGF-β1) (clone TW7-16B4) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom). This was followed by intracellular staining with FOXP3 Alexa Fluor® 647. Data shown was generated by gating on CD4+ lymphocyte population.
  • TW7-16B4_BV421_LAP_Antibody_FC_1_091912
    C57BL/6 mouse splenocytes were stimulated with CD3, CD28, and recombinant mouse IL-2 for 48 hours, then surface stained with CD4 FITC and LAP (TGF-β1) (clone TW7-16B4) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom). This was followed by intracellular staining with FOXP3 Alexa Fluor® 647. Data shown was generated by gating on CD4+ lymphocyte population.
  • TW7-16B4_BV421_LAP_Antibody_FC_2_091912
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability
141407 125 µL $215.00
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141408 50 µg $242.00
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Description

Transforming growth factor beta (TGF-β) is a cytokine that has critical functions in the immune response by regulating Treg and Th17 cells. TGF-β is first synthesized as pro-TGF-β and then it is cleaved by furin proprotein convertase in the Golgi apparatus to produce the dimeric propeptides called latency-associate peptide (LAP) that non-covalently associates with the dimeric mature TGF-β to prevent its activity. This complex can further associate with latent-TGF-β-binding protein (LTBP) to produce a large latent form for deposition onto the extracellular matrix. The latent-TGF-β can be expressed on the membrane of activated Treg cells, immature dendritic cells, megakaryocytes, and platelets.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse Tgfb1-transduced P3U1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone TW7-16B4 has been reported to not cross-react with bovine LAP.2 Several anti-LAP antibody clones have been compared and characterized for their LAP reactivity.2 TW7-16B4 recognizes recombinant LAP, latent TGF-β, and pro-TGF-β.

Additional reported applications (for relevant formats) include: Western blotting1 and immunoprecipitation1. 

Application References

(PubMed link indicates BioLegend citation)
  1. Oida T, et al. 2010. PLoS One 5:e15523. (FC, IP, WB)
  2. Oida T, et al. 2011. PLoS One 6:e18365. (Neut)
Product Citations
  1. Liang X, et al. 2022. Cancer Commun (Lond). 42:205. PubMed
  2. Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
  3. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  4. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  5. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  6. De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
  7. Bachy S, et al. 2022. iScience. 25:103758. PubMed
  8. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  9. Zheng G, et al. 2017. Respir Res. 10.1186/s12931-017-0599-5. PubMed
  10. Prakash MD, et al. 2021. PLoS One. 16:e0247492. PubMed
  11. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  12. Culina S, et al. 2015. Diabetes. 64: 3532 - 3542. PubMed
  13. L M, et al. 2016. Brain. 139: 1939-1957. PubMed
  14. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
RRID
AB_2650898 (BioLegend Cat. No. 141407)
AB_2650898 (BioLegend Cat. No. 141408)

Antigen Details

Structure
Dimmers of latency-associated peptide non-covalently associated with dimmers of mature TGF-β
Distribution

Many cell types, highly expressed on activated Tregs and platelets

Function
TGF-β controls cell differentiation, tissue morphogenesis, cell growth, inflammation, matrix synthesis, apoptosis, and regulates immune response
Ligand/Receptor
TGF-β receptors
Cell Type
Dendritic cells, Platelets, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Oida T, et al. 2010. PLoS One 5:e15523.
2. Tran D, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.
3. Ochi H, et al. 2006. Nat. Med. 12:627.
4. Oida T, et al. 2003. J. Immunol. 170:2516.
5. Nakamura K. 2001. J. Exp. Med. 194:629.
6. Miyazono K, et al. 1993. Growth Factors 8:11.

Gene ID
21803 View all products for this Gene ID
UniProt
View information about LAP on UniProt.org
Go To Top Version: 2    Revision Date: 08/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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