- Clone
- W20104A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CEACAM8
- Isotype
- Rat IgG2a, κ
-
Human peripheral blood granulocytes were stained with anti-human CD66b (clone W20104A) Brilliant Violet 510™ (filled histogram) or rat IgG2a, κ Brilliant Violet 510™ isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 386809 | 25 tests | $193.00 | |||
CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, which is expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Full length transfectant RBL1 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- RRID
-
AB_3662421 (BioLegend Cat. No. 386809)
Antigen Details
- Structure
- Ig superfamily, CEA antigen group, GPI-linked glycoprotein
- Distribution
-
Granulocytes
- Function
- Cell adhesion, neutrophil activation
- Ligand/Receptor
- CD66c
- Cell Type
- Granulocytes
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Kuijpers T, et al. 1993. J Immunol. 151:4934-40.
- Kuroki M, et al. 1992. J Leuk Biol. 52:551-7.
- Gene ID
- 1088 View all products for this Gene ID
- UniProt
- View information about CD66b on UniProt.org
Other Formats
View All CD66b Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD66b | W20104A | FC |
| Brilliant Violet 510™ anti-human CD66b | W20104A | FC |
| Brilliant Violet 711™ anti-human CD66b | W20104A | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD66b
Human peripheral blood granulocytes were stained with anti-h... -
Brilliant Violet 510™ anti-human CD66b
Human peripheral blood granulocytes were stained with anti-h... -
Brilliant Violet 711™ anti-human CD66b
Human peripheral blood granulocytes were stained with anti-h...