Brilliant Violet 510 anti-mouse CD90.2 (Thy1.2) Antibody anti-CD90.2

Pricing & Availability

Clone: 30-H12 (See other available formats)
Regulatory Status: RUO
Other Names: Thy-1.2
Isotype: Rat IgG2b, κ

30-H12_BV510_CD90point2_Antibody_120415 — C57BL/6 thymocytes were stained with CD90.2 (clone 30-H12) Brilliant Violet 510™ (filled histogram) or rat IgG2b, κ Brilliant Violet 510™ isotype control (open histogram).

Compare all formats See Brilliant Violet 510™ spectral data

Cat #

Size

Price

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105335

50 µg

$297.00

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Description

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca²⁺ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.

Technical data sheet

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse thymus or spleen

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion^1,2,7, costimulation of CD3/TCR-mediated signal transduction^3,4, and immunohistochemical staining⁵ of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301 & 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 105351 & 105352).

Application References (PubMed link indicates BioLegend citation)

Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
Seaman WE. 1983. J. Immunol. 130:1713. (Deplete)
Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim)
Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim)
Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC)
Hardy B, et al. 2005. Int. Immunol. 17:615.
Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
Sungur CM, et al. 2013. PNAS. 110:7401. PubMed

Product Citations

Wang D, et al. 2018. Immunity. 48:659. PubMed
Bai Y, et al. 2021. Front Cell Dev Biol. 9:730095. PubMed
Tanaka Y, et al. 2020. Sci Rep. 10:17284. PubMed
Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed

RRID

AB_2566587 (BioLegend Cat. No. 105335)

Antigen Details

Structure: Ig superfamily, 25-35 kD
Distribution: Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice
Function: Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor: CD45
Cell Type: Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Craig W, et al. 1993. J. Exp. Med. 177:1331.
3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1.
4. Mayani H, et al. 1994. Blood 83:2410.
Gene ID: 21838 View all products for this Gene ID
UniProt: View information about CD90.2 on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Other Formats

View All CD90.2 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-mouse CD90.2 (Thy1.2)	30-H12	FC
FITC anti-mouse CD90.2 (Thy1.2)	30-H12	FC
PE anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Purified anti-mouse CD90.2 (Thy1.2)	30-H12	FC,IHC-F,Costim,Depletion
APC anti-mouse CD90.2 (Thy1.2)	30-H12	FC
PE/Cyanine5 anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Alexa Fluor® 488 anti-mouse CD90.2 (Thy1.2)	30-H12	FC,IHC-F
Alexa Fluor® 647 anti-mouse CD90.2 (Thy1.2)	30-H12	FC,IHC-F
Alexa Fluor® 700 anti-mouse CD90.2 (Thy1.2)	30-H12	FC
PerCP anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Pacific Blue™ anti-mouse CD90.2 (Thy1.2)	30-H12	FC
PE/Cyanine7 anti-mouse CD90.2 (Thy1.2)	30-H12	FC
APC/Cyanine7 anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Brilliant Violet 570™ anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Brilliant Violet 785™ anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Purified anti-mouse CD90.2 (Thy1.2) (Maxpar® Ready)	30-H12	FC,CyTOF®
Brilliant Violet 510™ anti-mouse CD90.2 (Thy1.2)	30-H12	FC
PerCP/Cyanine5.5 anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Brilliant Violet 605™ anti-mouse CD90.2 (Thy1.2)	30-H12	FC
PE/Dazzle™ 594 anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Brilliant Violet 421™ anti-mouse CD90.2 (Thy1.2)	30-H12	FC
TotalSeq™-A0075 anti-mouse CD90.2 (Thy1.2)	30-H12	PG
APC/Fire™ 750 anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Brilliant Violet 711™ anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Ultra-LEAF™ Purified anti-mouse CD90.2 (Thy1.2)	30-H12	FC,Depletion,Costim,IHC
TotalSeq™-C0075 anti-mouse CD90.2 (Thy1.2)	30-H12	PG
TotalSeq™-B0075 anti-mouse CD90.2 (Thy1.2)	30-H12	PG
PE/Fire™ 810 anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Spark Blue™ 574 anti-mouse CD90.2 (Thy1.2) (Flexi-Fluor™)	30-H12	FC
Spark Red™ 718 anti-mouse CD90.2 (Thy1.2) (Flexi-Fluor™)	30-H12	FC

Go To Top Version: 1 Revision Date: 12/10/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.