Brilliant Violet 570™ anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
5.1H11 (See other available formats)
Regulatory Status
RUO
Other Names
Leu-19, NKH1, NCAM-1
Isotype
Mouse IgG1, κ
5.1H11_BV570_CD56_Antibody_FC_071615
Human peripheral blood lymphocytes were stained with CD56 (clone 5.1H11) Brilliant Violet 570™ (filled histogram) or mouse IgG1, κ Brilliant Violet 570™ isotype control (open histogram).
  • 5.1H11_BV570_CD56_Antibody_FC_071615
    Human peripheral blood lymphocytes were stained with CD56 (clone 5.1H11) Brilliant Violet 570™ (filled histogram) or mouse IgG1, κ Brilliant Violet 570™ isotype control (open histogram).
Compare all formats See Brilliant Violet 570™ spectral data
Cat # Size Price Quantity Check Availability
362539 25 tests $209.00
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362540 100 tests $429.00
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Description

CD56 is a single transmembrane glycoprotein also known as NCAM (neural cell adhesion molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK and NKT cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparan sulfate.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human myotube cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 570™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 570™ excites at 405 nm and emits at 570 nm. The bandpass filter 585/42 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 570™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Walsh FS, et al. 1981. Nature 289:60. (FC)
  2. Pavlath GK, et al. 1986. J. Cell Biol. 102:124. (FC)
  3. Pavlath GK, et al. 1989. Nature 337:570. (FC)
  4. Pulido R, et al. 1988. J. Immunol. 140:3851. (FC)
Product Citations
  1. Saheb Sharif-Askari F, et al. 2023. Sci Rep. 17344:13. PubMed
  2. Berezhnoy A, et al. 2020. Cell Rep Med. 1:100163. PubMed
RRID
AB_2565917 (BioLegend Cat. No. 362539)
AB_2565917 (BioLegend Cat. No. 362540)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparan sulfate
Antigen References

1. Lanier L, et al. 1991. J. Immunol. 146:4421
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71
3. Cremer H, et al. 1994. Nature 367:455.

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org

Other Formats

View All CD56 (NCAM) Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD56 (NCAM) 5.1H11 FC
APC anti-human CD56 (NCAM) 5.1H11 FC
PerCP/Cyanine5.5 anti-human CD56 (NCAM) 5.1H11 FC
PE anti-human CD56 (NCAM) 5.1H11 FC
PE/Cyanine7 anti-human CD56 (NCAM) 5.1H11 FC
APC/Cyanine7 anti-human CD56 (NCAM) 5.1H11 FC
PE/Cyanine5 anti-human CD56 (NCAM) 5.1H11 FC
Alexa Fluor® 647 anti-human CD56 (NCAM) 5.1H11 FC
Alexa Fluor® 488 anti-human CD56 (NCAM) 5.1H11 FC
Pacific Blue™ anti-human CD56 (NCAM) 5.1H11 FC
Alexa Fluor® 700 anti-human CD56 (NCAM) 5.1H11 FC
PerCP anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 650™ anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 510™ anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 421™ anti-human CD56 (NCAM) 5.1H11 FC
Biotin anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 605™ anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 570™ anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 711™ anti-human CD56 (NCAM) 5.1H11 FC
PE/Dazzle™ 594 anti-human CD56 (NCAM) 5.1H11 FC
FITC anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 785™ anti-human CD56 (NCAM) 5.1H11 FC
Ultra-LEAF™ Purified anti-human CD56 (NCAM) 5.1H11 FC
APC/Fire™ 750 anti-human CD56 (NCAM) 5.1H11 FC
PE anti-human CD56 (NCAM) 5.1H11 FC
APC anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 750™ anti-human CD56 (NCAM) 5.1H11 FC
TotalSeq™-A0047 anti-human CD56 (NCAM) 5.1H11 PG
TotalSeq™-C0047 anti-human CD56 (NCAM) 5.1H11 PG
TotalSeq™-B0047 anti-human CD56 (NCAM) 5.1H11 PG
Spark NIR™ 685 anti-human CD56 (NCAM) 5.1H11 FC
KIRAVIA Blue 520™ anti-human CD56 (NCAM) 5.1H11 FC
GMP PE anti-human CD56 (NCAM) 5.1H11 FC
TotalSeq™-D0047 anti-human CD56 (NCAM) 5.1H11 PG
GMP APC anti-human CD56 (NCAM) 5.1H11 FC
Spark YG™ 593 anti-human CD56 (NCAM) 5.1H11 FC
Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD56 (NCAM) SF 5.1H11 FC
Spark Red™ 718 anti-human CD56 (NCAM) 5.1H11 FC
Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD56 (NCAM) SF 5.1H11 FC
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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