Brilliant Violet 605™ anti-mouse CD117 (c-kit) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Murine IL-3 dependent mast cells

Formulation

µl sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.

Concentration

µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Injection of ACK2 in mice of NOD genetic background is not recommended as mice develop immediate anaphylaxis, resulting in animal death. Treatment is possible with co-injection of Benadryl.³

ACK2 has been reported to block c-Kit function. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 135103). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 135114) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)

1. Yoshinaga K, et al. 1991. Development 113:689.
2. Broudy VC, et al. 1996. Blood 88:75.
3. Louvet C, et al. 2008. Proc. Natl. Acad. Sci. USA 105:18895.

Product Citations

1. Oetjen LK et al. 2017. Cell. 171(1):217-228 . PubMed
2. Chen S, et al. 2022. Cancer Discov. :. PubMed
3. Benhamou D, et al. 2018. Front Immunol. 2.260416667. PubMed
4. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
5. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
6. Green DP, et al. 2019. Neuron. 101:412. PubMed
7. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
8. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
9. Wang F, et al. 2021. Cell. 184(2):422-440.e17. PubMed
10. Gentek R, et al. 2018. Immunity. 48:1160. PubMed
11. Filtjens J, et al. 2021. Nat Commun. 12:2936. PubMed

RRID

AB_2562040 (BioLegend Cat. No. 135121)
AB_2562040 (BioLegend Cat. No. 135120)
AB_2562040 (BioLegend Cat. No. 135122)

Antigen Details

Structure

Ig superfamily, 145 kD

Distribution

Hematopoietic stem cells, AML, mast cells, oocytes, interstitial theca cells, leydig cells

Function

Growth factor receptor, tyrosine kinase

Ligand/Receptor

Stem Cell Factor (SCF)

Cell Type

Embryonic Stem Cells, Mesenchymal Stem Cells

Biology Area

Immunology, Stem Cells

Molecular Family

CD Molecules

Antigen References

1. Barclay A et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ. et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P et al. 1986. Nature 320:415.
5. Witte ON. et al. 1990. Cell 63:5.

Gene ID

16590 View all products for this Gene ID

UniProt

View information about CD117 on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Other Formats