Brilliant Violet 650™ anti-mouse CD226 (DNAM-1) Antibody

Pricing & Availability
Clone
TX42.1 (See other available formats)
Regulatory Status
RUO
Other Names
DNAM-1, PTA1 (Platelet and T cell activation antigen 1), TLISA1, LFA-1 associated molecule PTA-1
Isotype
Rat IgG2a, κ
TX42dot1_BV650_CD226_Antibody_1_083017
C57BL/6 mouse splenocytes were stained with CD8 FITC and CD226 (clone TX42.1) Brilliant Violet 650™ (top) or Rat IgG2a, κ Brilliant Violet 650™ isotype control (bottom).
  • TX42dot1_BV650_CD226_Antibody_1_083017
    C57BL/6 mouse splenocytes were stained with CD8 FITC and CD226 (clone TX42.1) Brilliant Violet 650™ (top) or Rat IgG2a, κ Brilliant Violet 650™ isotype control (bottom).
  • TX42dot1_BV650_CD226_Antibody_2_083017
Compare all formats See Brilliant Violet 650™ spectral data
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133621 50 µg $303.00
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Description

Mouse CD226 (DNAM-1) is a 65 kd glycoprotein of a member of Ig super-family adhesion molecule containing 2 immunoglobulin-like domains. It is associated with leukocyte function associated antigen-1 (LFA-1) involving in TCR-mediated signal transduction for T cell proliferation and differentiation. CD226 is also involved in NK cell and T cell mediated cytotoxicity against certain tumors. CD226 (DNAM-1) is constitutively expressed on naïve CD8+ and CD4+ T cells, macrophages, and NK cells. It was reported that CD226 expression is up-regulated on Th1 cells, but down-regulated on Th2 and Th0 cells. CD155 and CD112 are the ligands for CD226.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse DNAM-1 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Dardalhon V, et al. 2005. J. Immunol. 175:1558
  2. Shirakawa J, et al. 2006. International Immunology 18(6):951-957
RRID
AB_2716083 (BioLegend Cat. No. 133621)

Antigen Details

Structure
65 kd glycoprotein of a member of Ig super-family containing 2 immunoglobulin-like domains.
Distribution

Expressed on majority of CD8+ and CD4+ T cells, NK cells, monocytes/macrophages and a subset of B cells. Reported to up-regulated on Th1 cells, but down-regulated in Th2 cells.

Function
Mediate cellular adhesion, involved in LFA-1 co-stimulatory signal for T cell differentiation and proliferation.
Ligand/Receptor
CD112 and CD155
Cell Type
B cells, Macrophages, Monocytes, NK cells, T cells, Th1
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1 Tahara Hanaoka S, et al. 2006. Blood. 107:1491
2 Shibuya K et al. 2003. J. Exp. Med. 198:1829.

Gene ID
225825 View all products for this Gene ID
UniProt
View information about CD226 on UniProt.org
Go To Top Version: 0    Revision Date: 08/30/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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