- Regulatory Status
- RUO
- Other Names
- SAv-Cyanine5, SAV
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Unpassaged live mouse primary retinal pigment epithelial cells engulfed biotinylated photocreceptor outer segments (POS) coupled to Cyanine5 Streptavidin (red). Cells were subsequently stained with Flash Phalloidin™ Green 488 (green) and DAPI (blue). Image generously submitted to the 2017 Cell Life Imaging Competition by Francesca Mazzoni from Fordham University.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 405209 | 100 µg | $206.00 | |||
Streptavidin binds to biotin with high affinity. Streptavidin-Cyanine5 is useful for detecting biotinylated antibodies. The excitation of Cyanine5 by 625-650 nm laser light induces a fluorescence emission maximum of 670 nm.
Product Details
- Verified Reactivity
- Human, Mouse, Rat, All Species
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- Streptavidin is conjugated with Cyanine5 under optimal conditions.
- Concentration
- 0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
- Storage & Handling
- The streptavidin-Cyanine5 solution should be stored undiluted between 2°C and 8°C, and protected from exposure to light. Do not freeze.
- Application
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FC - Quality tested
ICFC - Verified
- Recommended Usage
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Each lot of this Streptavidin-Cyanine5 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Cyanine5 conjugation. For immunofluorescent staining, the recommended use of this reagent is = 0.5 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Streptavidin-Cyanine5 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of streptavidin-Cyanine5 is 60 kD and Streptavidin alone is 52 kD.
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
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Antigen Details
- Gene ID
- NA
- UniProt
- View information about Biotin on UniProt.org