Direct-Blot™ HRP anti-Bcl-2 Antibody

Pricing & Availability
Clone
BCL/10C4 (See other available formats)
Regulatory Status
RUO
Other Names
B-cell leukemia 2
Isotype
Mouse IgG1, κ
BCLslash10C4_DB-HRP_Bcl-2_Antibody_1_090718_updated
Western blot analysis of 15 µg cell lysates from Mouse (Lane 1) and Rat (Lane 2) Spleen cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 1 µg/ml Direct-Blot™ anti-Bcl-2 antibody, clone BCL/10C4. Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 664804, 1:25000 dilution). Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
  • BCLslash10C4_DB-HRP_Bcl-2_Antibody_1_090718_updated
    Western blot analysis of 15 µg cell lysates from Mouse (Lane 1) and Rat (Lane 2) Spleen cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 1 µg/ml Direct-Blot™ anti-Bcl-2 antibody, clone BCL/10C4. Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 664804, 1:25000 dilution). Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
Compare all formats
Cat # Size Price Quantity Check Availability
633513 25 µL $101.00
 

This product is no longer available.
633514 100 µL $340.00
 

This product is no longer available.
Description

Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Two reported isoforms exist α=25 kD; β=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, the nuclear envelope, and the endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-xL, and Bag-1. Clone BCL/10C4 has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence of the mouse and rat Bcl-2 protein.

Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
N-terminal, Amino acid residues 61-76 of mouse Bcl-2
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per mL (1:100 - 1:1000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue4, immunocytochemical staining5, and immunoprecipitation5.

Application References

(PubMed link indicates BioLegend citation)
  1. Hsu YT, et al. 1997. J. Biol. Chem. 272:13829.
  2. Zuber J, et al. 2011. Genes Dev. 25:1628. (WB) PubMed
  3. Doi K, et al. 2012. Biochem Biophys Res Commun. 425:107. PubMed
  4. Yamanaka, et al. 2003. J. Immunol. 170:816. (IHC)
  5. Conus S, et al. 2000. EMBO J. 19:1534. (WB, IP, ICC)
RRID
AB_2750245 (BioLegend Cat. No. 633513)
AB_2750245 (BioLegend Cat. No. 633514)

Antigen Details

Structure
Apoptosis regulator proteins, Bcl-2 family, BH1-4 domains. Homo- or hetero-dimer; isoforms α, β 25 kD, 22 kD
Distribution

Outer mitochondrial membrane, intracellular membrane nuclear envelope, endoplasmic reticulum

Function
Blocks apoptotic death by controlling the mitochondrial membrane permeability. Converted to pro-apoptotic activity by cleavage of BH4 domain. Regulates cell cycle progression via ROS
Modification
Phosphorylation, Ubiquitination
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Antigen References

1. Tsujimoto Y, et al. 1986 P. Natl. Acad. Sci. USA 83:5214.
2. Yang E, et al. 1995. Cell 80:285.
3. Huang Z, et al. 2000. Oncogene 19:6627.
4. Deng X, et al. 2003. Blood. 102:3179.

Regulation
Phosphorylation by ASK1/JNK1, PKC, ERKs, stress-activated kinases
Gene ID
596 View all products for this Gene ID
UniProt
View information about Bcl-2 on UniProt.org
Go To Top Version: 1    Revision Date: 09/10/2018

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account