Granzyme B is a member of the granzyme family of serine proteases found specifically in granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Perforin/granzyme pathway is a molecular weapon for the defense against virus-infected and malignantly transformed host cells. Perforin protein creates a pore in the membrane of targets cells and facilitate the entry of granzymes. This results in results in the translocation of Granzyme B into the nucleus and initiation of apoptosis. Other granzymes have also been implicated in apoptosis, but Granzyme B appears to be the most potent. Human Granzyme B is a potential immunoconjugate and an important facilitator in chronic inflammation and wound healing.
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Sensitivity
0.5 pg/mL
Standard Range
200 - 3.1 pg/mL
Materials Not Included
Microwell plates: BioLegend Cat. No. 423501 is recommended
Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
Plate Sealers: BioLegend Cat. No. 423601 is recommended
PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4 , 0.2 g KH2PO4 , 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
Deionized (DI) water
A microplate reader capable of measuring absorbance at 450 nm
Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
