Mouse Granulocyte-Colony Stimulating Factor (G-CSF), also known as mouse CSF-3, is a growth factor and pleiotropic cytokine that influences cells from the bone marrow to differentiate, proliferate and activate the neutrophilic granulocyte lineage (Neutrophils). Mouse G-CSF is produced primarily by monocytes and macrophages upon activation and is measured and tested for differentiation studies. Mouse G-CSF belongs to the IL-6 superfamily and is a polypeptide chain containing 179 amino acids and has a molecular weight of 19 kD. It is naturally produced by the body and is also available as an injection. Human and mouse G-CSF proteins share 76% sequence identity at the amino acid level.
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Sensitivity
3.8 - 8.6 pg/mL
Standard Range
12.5 - 800 pg/mL
Materials Not Included
Microwell plates: BioLegend Cat. No. 423501 is recommended
Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
Plate Sealers: BioLegend Cat. No. 423601 is recommended
PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
Deionized (DI) water
A microplate reader capable of measuring absorbance at 450 nm
Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
