ELISA MAX™ Deluxe Set Mouse M-CSF

Pricing & Availability
Regulatory Status
RUO
Other Names
colony stimulating factor 1 (macrophage), CSF1, CSF-1, Lanimostim, macrophage colony stimulating factor, macrophage colony-stimulating factor 1, MCSF, M-CSF, MCSFlanimostim, MGC31930
ELISA-MAX_Deluxe_Mouse_M-CSF_082421.png
  • ELISA-MAX_Deluxe_Mouse_M-CSF_082421.png
Cat # Size Price Quantity Check Availability
448504 5 plates $305.00
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Description

As a potent hematopoietic cytokine, M-CSF is involved in diverse processes, such as regulation of atherosclerosis or brain, bone resorption, placental development, and inflammatory responses. M-CSF (macrophage colonystimulating factor) is a potent lineage restricted hematopoietic growth factor and member of the CSF family of hormone-like glycoproteins. It plays a key role in the regulation of cellular undifferentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells.

BioLegend’s ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The kit contains a monoclonal capture antibody and biotinylated monoclonal antibody specific for mouse M-CSF. BioLegend’s ELISA MAX™ Deluxe Set contains the components necessary for the accurate quantification of natural and recombinant mouse M-CSF. The set is designed for cost-effective and accurate quantification of mouse M-CSF in cell culture supernatant, serum, plasma or other biological fluids. BioLegend’s ELISA MAX™ Deluxe Sets are sensitive, accurate, and robust.

Technical data sheet

Kit Contents

Kit Contents
  • Mouse M-CSF ELISA MAX™ Capture Antibody (200X)
  • Mouse M-CSF ELISA MAX™ Detection Antibody (200X)
  • Mouse M-CSF Standard
  • Avidin-HRP (1000X)
  • Substrate Solution D
  • Coating Buffer B (5X)
  • Assay Diluent B (5X)

Product Details

Verified Reactivity
Mouse
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
3.52 - 4.50 pg/mL
Standard Range
15.6 - 1000 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20). BioLegend Cat. No. 421601 is recommended.
  • Stop Solution (e.g. 2N H2SO4) BioLegend Cat. No. 423001 is recommended
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Cell Proliferation and Viability, Immunology
Molecular Family
Cytokines/Chemokines, Growth Factors
Gene ID
NA
UniProt
View information about M-CSF on UniProt.org
Go To Top Version: 2    Revision Date: 09/20/2022

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