- Regulatory Status
- RUO
- Other Names
- colony stimulating factor 1 (macrophage), CSF1, CSF-1, Lanimostim, macrophage colony stimulating factor, macrophage colony-stimulating factor 1, MCSF, M-CSF, MCSFlanimostim, MGC31930
Cat # | Size | Price | Quantity Check Availability | ||
---|---|---|---|---|---|
448504 | 5 plates | $305.00 |
As a potent hematopoietic cytokine, M-CSF is involved in diverse processes, such as regulation of atherosclerosis or brain, bone resorption, placental development, and inflammatory responses. M-CSF (macrophage colonystimulating factor) is a potent lineage restricted hematopoietic growth factor and member of the CSF family of hormone-like glycoproteins. It plays a key role in the regulation of cellular undifferentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells.
BioLegend’s ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The kit contains a monoclonal capture antibody and biotinylated monoclonal antibody specific for mouse M-CSF. BioLegend’s ELISA MAX™ Deluxe Set contains the components necessary for the accurate quantification of natural and recombinant mouse M-CSF. The set is designed for cost-effective and accurate quantification of mouse M-CSF in cell culture supernatant, serum, plasma or other biological fluids. BioLegend’s ELISA MAX™ Deluxe Sets are sensitive, accurate, and robust.
Kit Contents
- Kit Contents
-
-
- Mouse M-CSF ELISA MAX™ Capture Antibody (200X)
- Mouse M-CSF ELISA MAX™ Detection Antibody (200X)
- Mouse M-CSF Standard
- Avidin-HRP (1000X)
- Substrate Solution D
- Coating Buffer B (5X)
- Assay Diluent B (5X)
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Product Details
- Verified Reactivity
- Mouse
- Application
-
ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Sensitivity
- 3.52 - 4.50 pg/mL
- Standard Range
- 15.6 - 1000 pg/mL
- Materials Not Included
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- Microwell plates: BioLegend Cat. No. 423501 is recommended
- Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20). BioLegend Cat. No. 421601 is recommended.
- Stop Solution (e.g. 2N H2SO4) BioLegend Cat. No. 423001 is recommended
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
- Deionized (DI) water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
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Antigen Details
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Immunology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Gene ID
- NA
- UniProt
- View information about M-CSF on UniProt.org