FITC anti-human CD209 (DC-SIGN) Antibody

Pricing & Availability
Clone
9E9A8 (See other available formats)
Regulatory Status
RUO
Other Names
Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin
Isotype
Mouse IgG2a, κ
9E9A8_FITC_071608.jpg
Human monocytes-derived dendritic cells stained with 9E9A8 FITC
  • 9E9A8_FITC_071608.jpg
    Human monocytes-derived dendritic cells stained with 9E9A8 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability
330103 25 tests $106.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
330104 100 tests $229.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD209, known as Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin (DC-SIGN), is a 44 kD type II transmembrane glycoprotein and a member of the C-type lectin family. CD209 is expressed on myeloid dendritic cells, placental macrophages, liver and placental endothelial cells. CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTN2A1), and mediates dendritic cell migration and T cell proliferation. Importantly, CD209 is a receptor of HIV-1 and some other viruses (such as West Nile virus, hepatitis C virus, etc), and some bacteria or parasites. It plays a criti­cal role in capturing and internalizing those pathogens. LSP1 (leukocyte-specific protein 1) interacts with the cytoplasmic domain of CD209 and mediates transport of HIV to the proteasome.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular domain of human DC-SIGN
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry on frozen tissue sections1 and spatial biology (IBEX)2,3.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Granelli-Piperno A, et al. 2005. J Immunol. 175:4265. (IHC-F)
  2. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  3. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Rosell-Mases E, et al. 2023. Nat Commun. 7770:14. PubMed
  2. Zhang Z, Shively J 2013. PLoS One. 8:67649. PubMed
  3. Su R, et al. 2020. Cancer Cell. 38(1):79-96.e11. PubMed
  4. Carrion J, et al. 2012. J Immunol. 189:3178. PubMed
  5. Rodriguez-Pla A, et al. 2014. J Immunol. 192:5586. PubMed
RRID
AB_1134048 (BioLegend Cat. No. 330103)
AB_1134048 (BioLegend Cat. No. 330104)

Antigen Details

Distribution

Dendritic cells

Cell Type
Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Granelli-Piperno A, et al. 2005. J Immunol. 175:4265.

Gene ID
30835 View all products for this Gene ID
UniProt
View information about CD209 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account