FITC anti-human CD272 (BTLA) Antibody

Pricing & Availability
Clone
MIH26 (See other available formats)
Regulatory Status
RUO
Other Names
BTLA, B and T lymphocyte attenuator
Isotype
Mouse IgG2a, κ
MIH26_FITC_CD272_Antibody_1_100517
Human peripheral blood lymphocytes were stained with CD19 Brilliant Violet 421™ and anti-human CD272 (clone MIH26) FITC (top) or Mouse IgG2a, κ FITC isotype control (bottom).
  • MIH26_FITC_CD272_Antibody_1_100517
    Human peripheral blood lymphocytes were stained with CD19 Brilliant Violet 421™ and anti-human CD272 (clone MIH26) FITC (top) or Mouse IgG2a, κ FITC isotype control (bottom).
  • MIH26_FITC_CD272_Antibody_2_100517
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability
344523 25 tests $136.00
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344524 100 tests $370.00
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Description

B and T lymphocyte attenuator (BTLA) is an Ig superfamily coinhibitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4. BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand Herpes Virus Entry Mediator (HVEM) is critical for negatively regulating immune response. The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8+ memory T cell population, increased severity of colitis, and reduced effectiveness of T regulatory cells. BTLA plays an important role in the induction of peripheral tolerance of both CD4+ and CD8+ T cells in vivo. Tolerant T cells have significant up-regulated expression of BTLA compared with effector and naïve T cells. BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity. It has been reported that BTLA may regulate T cell function through binding to B7-H4.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human BTLA transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inhibition of T cell proliferation and cytokine production1. Clone MIH26 has agonistic activity on BTLA, resulting in the inhibition of activation.

The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for use in in vitro and in vivo biofunctional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Otsuki N, et al. 2006. Biochem. Bioph. Res. Co. 344:1121.
  2. Okano M, et al. 2008. Clin. Exp. Allergy 38:1891.
Product Citations
  1. Sun W, et al. 2023. Nature. 614:309. PubMed
RRID
AB_2716232 (BioLegend Cat. No. 344523)
AB_2716232 (BioLegend Cat. No. 344524)

Antigen Details

Structure
Ig superfamily, with similar structure to CTLA-4 and PD-1
Distribution

B cells and T lymphocytes, NK cells and dendritic cells

Function
Negative regulation of T cell activation, proliferation and cytokine production
Ligand/Receptor
HVEM
Cell Type
B cells, Dendritic cells, NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Watanabe N, et al. 2003. Nat. Immunol. 4:670.
2. Sun Y, et al. 2009. J. Immunol. 183:1946.
3. Gonzalez LC, et al. 2005. P. Natl. Acad. Sci. USA 102:1116.

Gene ID
151888 View all products for this Gene ID
UniProt
View information about CD272 on UniProt.org
Go To Top Version: 0    Revision Date: 10/10/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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