FITC anti-human CD34 Antibody

Pricing & Availability
Clone
581 (See other available formats)
Regulatory Status
RUO
Workshop
V MA27
Other Names
Gp105-120, My10
Isotype
Mouse IgG1, κ
A_581_FITC_072909.jpg
Human peripheral blood mononuclear cells stained with 581 FITC (lower panel) or FITC mouse IgG1 isotype control (upper panel) and CD45 (HI30) PerCP (gated on CD14- cell population)
  • A_581_FITC_072909.jpg
    Human peripheral blood mononuclear cells stained with 581 FITC (lower panel) or FITC mouse IgG1 isotype control (upper panel) and CD45 (HI30) PerCP (gated on CD14- cell population)
  • B_581_FITC_072909.jpg
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability
343503 25 tests $102.00
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343504 100 tests $226.00
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Description

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press.
  2. Felschow DM, et al. 2001. Blood 97:3768.
  3. Rudin CE, et al. 1997. Br. J. Haematol. 97:488.
  4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  5. Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC)
  6. Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)
Product Citations
  1. Bowley TY, et al. 2022. Cancer Res Commun. 2:1436. PubMed
  2. Filipello F, et al. 2023. Acta Neuropathol. 145:749. PubMed
  3. Ishiuchi N, et al. 2023. Stem Cell Res Ther. 14:121. PubMed
  4. Lütge M, et al. 2023. Nat Immunol. . PubMed
  5. Han X, et al. 2023. Stem Cells Int. 2023:8992284. PubMed
  6. Gan T, et al. 2021. Cell Rep. 37:109824. PubMed
  7. Benjamin DI, et al. 2022. Cell Metab. 34:902. PubMed
  8. Huang J, et al. 2022. Clin Exp Pharmacol Physiol. 49:881. PubMed
  9. Zhang T, et al. 2022. IUBMB Life. 74:1081. PubMed
  10. Wheeler MM, et al. 2022. Nat Commun. 13:7592. PubMed
  11. Fu H, et al. 2023. Regen Ther. 22:7. PubMed
  12. Chen Y, et al. 2023. Stem Cells Int. 2023:7482546. PubMed
  13. Chen X, et al. 2023. Exp Ther Med. 25:99. PubMed
  14. Wei X, et al. 2022. Mol Cell. 82:1225. PubMed
  15. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
  16. Vishnoi M, et al. 2018. Cancer Res. 78:5349. PubMed
  17. Vinjamur DS, et al. 2021. Nat Genet. 53:719. PubMed
  18. Padmanabhan U, et al. 2020. Eur J Haematol. 104:170. PubMed
  19. Casetti L, et al. 2013. Cancer Res. 73:2052. PubMed
  20. Tancharoen W, et al. 2019. Mol Med Rep. 19:5123. PubMed
  21. Sung SE, et al. 2021. Int J Mol Sci. 22:. PubMed
  22. Sung SE, et al. 2021. Vet Sci. 8:. PubMed
  23. Rao S, et al. 2021. Cell Stem Cell. 28(5):833-845.e5. PubMed
  24. Björklund &, et al. 2016. Nat Immunol. 17:451-460. PubMed
  25. Hansen IS, et al. 2018. Nat Commun. 9:863. PubMed
  26. Wu J, et al. 2021. Front Immunol. 12:601611. PubMed
  27. Sung SE, et al. 2021. Asian Spine J. Online ahead of print.. PubMed
  28. Sarkar TJ, et al. 2020. Nat Commun. 11:1545. PubMed
  29. Judson RN et al. 2018. Cell stem cell. 22(2):177-190 . PubMed
  30. Kerr BA, et al. 2021. Experimental Cell Research. 399(2):112456. PubMed
  31. Santos J, et al. 2013. J Transl Med. 11:18. PubMed
  32. Yan K, et al. 2022. iScience. 25:104822. PubMed
  33. Quarta M, et al. 2016. Nat Biotechnol. 10.1038/nbt.3576. PubMed
  34. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  35. Xu H, et al. 2022. Cell Death Dis. 13:478. PubMed
  36. Zeng J, et al. 2020. Nat Med. 26:535. PubMed
  37. Chruewkamlow N, et al. 2021. Stem Cell Res Ther. 12:520. PubMed
  38. Fathy El-Metwaly N, et al. 2021. Asian Pac J Cancer Prev. 22:3237. PubMed
  39. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  40. Ran Y, et al. 2022. Stem Cell Res Ther. 13:507. PubMed
  41. Busch A, et al. 2020. Int J Mol Sci. 21:. PubMed
  42. Xu L, et al. 2019. Haematologica. 10.3324/haematol.2018.207258. PubMed
  43. Vishnoi M, et al. 2019. Mol Oncol. 13(9): 1913. PubMed
  44. Matsuyama H, et al. 2019. Sci Rep. 9:13181. PubMed
  45. Lavaert M, et al. 2020. Immunity. 52(6):1088-1104. PubMed
  46. Pauken CM, et al. 2021. Cancers (Basel). 13:. PubMed
  47. Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed
  48. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  49. Sprouse ML, et al. 2019. Int J Mol Sci. 20:8. PubMed
  50. Zhang J, et al. 2021. Stem Cell Res Ther. 12:579. PubMed
RRID
AB_1731852 (BioLegend Cat. No. 343503)
AB_1731852 (BioLegend Cat. No. 343504)

Antigen Details

Structure
105-120 kD single chain mucin-like glycoprotein
Distribution

Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts

Function
Cell adhesion
Ligand/Receptor
L-selectin, E-selectin
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Krause DS, et al. 1996. Blood 87:1.
2. Puri KD, et al. 1995. J. Cell Biol. 131:261.
3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.

Gene ID
947 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org
Go To Top Version: 2    Revision Date: 03/18/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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