- Clone
- W19046A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-2, T cell growth factor (TCGF); Eosinophil differentiation factor (EDF); Killer cell helper factor (KHF); Macrophage-activating factor for cytotoxicity I (MAF-C I); Thymocyte differentiation factor (TDF)
- Isotype
- Rat IgG2b, κ
-
Human peripheral blood lymphocytes were stimulated with Cell Activation Cocktail (with Brefeldin A) (Cat. Nos. 423303 and 423304) for 6 hours, surface stained with anti-human CD3 PE, and then treated with the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). Cells were then stained with anti-human IL-2 (clone W19046A) FITC (left) or rat IgG2b, κ FITC isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 310607 | 25 tests | $125.00 | |||
| 310608 | 100 tests | $280.00 | |||
IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human IL-2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
- RRID
-
AB_3083300 (BioLegend Cat. No. 310607)
AB_3083300 (BioLegend Cat. No. 310608)
Antigen Details
- Structure
- Cytokine; 15.4 kD (Mammalian)
- Distribution
-
T cells
- Function
- Upregulated by NFAT; downregulated by TCF-8 and CIF (colostrums inhibitory factor)
- Ligand/Receptor
- High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603. - Gene ID
- 3558 View all products for this Gene ID
- UniProt
- View information about IL-2 on UniProt.org
Other Formats
View All IL-2 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human IL-2 | W19046A | ICFC |
| Spark Violet™ 423 anti-human IL-2 | W19046A | ICFC |
| APC anti-human IL-2 | W19046A | ICFC |
| FITC anti-human IL-2 | W19046A | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human IL-2
Human peripheral blood lymphocytes were stimulated with Cell... -
Spark Violet™ 423 anti-human IL-2
Human peripheral blood lymphocytes were stimulated with Cell... -
APC anti-human IL-2
Human peripheral blood lymphocytes were stimulated with Cell... -
FITC anti-human IL-2
Human peripheral blood lymphocytes were stimulated with Cell...