FITC anti-mouse CD357 (GITR) Antibody

Pricing & Availability
Clone
YGITR 765 (See other available formats)
Regulatory Status
RUO
Other Names
TNFRSF18, AITR
Isotype
Rat IgG2b, κ
YGITR_765_FITC_110805
C57BL/6 mouse splenocytes stained YGITR 765 FITC and CD25 APC
  • YGITR_765_FITC_110805
    C57BL/6 mouse splenocytes stained YGITR 765 FITC and CD25 APC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability
120205 50 µg $147.00
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Description

GITR, Glucocorticoid-induced TNFR-related gene, is a member of the TNF receptor superfamily, also known as TNFRSF18, and AITR (in humans). It is expressed at low levels on resting T lymphocytes and at high levels on CD4+CD25+ T regulatory (Treg) cells. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production, as well as MAPKs and NF-κB activation, and abrogate the inhibitory functions of CD4+CD25+ Treg cells. In vivo activation GITR causes development of autoimmune diseases and restores the suppressed immune responses.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse GITR protein and Transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 120216).

Application References

(PubMed link indicates BioLegend citation)
  1. Cobbold SP, et al. 2004. J. Immunol. 172:6003.
  2. Biburger M, et al. 2008. J. Leukocyte Biol. 84:264. PubMed
Product Citations
  1. Du J, et al. 2022. Sci Immunol. 7:eabo5407. PubMed
  2. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
RRID
AB_439723 (BioLegend Cat. No. 120205)

Antigen Details

Structure
TNF receptor superfamily member
Distribution

Low levels on resting T cells, high on CD4+CD25+ Treg cells, expression upregulated upon activation.

Function
Co-stimulate GITR expressing cells activation, proliferation, and cytokine production; inhibit the suppressive activities of CD4+CD25+ Treg cells.
Ligand/Receptor
GITR ligand (TNFSF18)
Cell Type
T cells, Tregs
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059
2. Ronchetti S, et al. 2004. Eur. J. Immunol. 34:613
3. Kanamaru F, et al. 2004. J. Immunol. 172:613
4. Shimizu J, et al. 2002. Nat. Immunol. 3:135
5. Stephens GL, et al. 2004. J. Immunol. 173:5008
6. McHugh RS, et al. 2002. Immunity 16:311

Gene ID
21936 View all products for this Gene ID
UniProt
View information about CD357 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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