- Clone
- DO-7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Tumor protein p53 (TP53)
- Isotype
- Mouse IgG2b, κ
-
A431 cells, which are a human epidermoid carcinoma cell line, was fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with p53 (clone DO-7) FITC (filled histogram) or mouse IgG2b, κ FITC isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 645803 | 25 tests | $129.00 | |||
| 645804 | 100 tests | $288.00 | |||
p53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- p53 N-terminal linear epitope aa 20-25
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Vojtesek B, et al. 1992. J. Immunol. Methods 151:237.
- Stephen CW, et al. 1995. J. Mol. Biol. 248:58.
- Product Citations
-
- RRID
-
AB_2561798 (BioLegend Cat. No. 645803)
AB_2561798 (BioLegend Cat. No. 645804)
Antigen Details
- Structure
- Tetramer; 53 kD
- Distribution
-
Nuclear when activated
- Function
- Tumor suppressor, transcription factor regulates cell cycle checkpoint, apoptosis, DNA integrity
- Interaction
- mdm2, mdmx, TopoI, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, HDAC1-3; mutant p53 associates with p63, p73
- Cell Type
- Mesenchymal Stem Cells
- Biology Area
- Cell Biology, DNA Repair/Replication, Stem Cells
- Gene ID
- 7157 View all products for this Gene ID
- UniProt
- View information about p53 on UniProt.org
Other Formats
View All p53 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-p53 | DO-7 | WB,ICC,ChIP,ICFC,ELISA |
| FITC anti-p53 | DO-7 | ICFC |
| PE anti-p53 | DO-7 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-p53
Total cell lysate from Raw264.7 (lane 1) and MCF-7 (lane 2) ... 
BT474 cells were stained with anti-p53 (clone DO-7), followe... 
Immunofluorescence of Daudi cells with (A) Mouse IgG2b, κ I... 
Whole cell extracts (15 µg total protein) from HEK293, A431,... 
Chromatin Immunoprecipitations (ChIP) were done with fixed a... 
THP-1 cells (negative control, open histogram), or Daudi cel... -
FITC anti-p53
A431 cells, which are a human epidermoid carcinoma cell line... -
PE anti-p53
A431 cells, which are a human epidermoid carcinoma cell line...