- Regulatory Status
- RUO
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Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with anti-human CD19 and FITC Recombinant Protein L (left) or stained with anti-human CD19 APC only (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 303653 | 50 tests | $225.00 | |||
Protein L was initially isolated from bacteria Peptostreptococcus magnus cell wall, and now is prepared as recombinant protein. Protein L has been found to have a high affinity for Ig κ light chains. This unique ability of protein L to bind to the κ light chains of antibodies makes it valuable in detection and purification of antibodies and antibody fragments that contain kappa light chains but not the Fc region, such as antigen-binding VL domains and Fv fragments prepared from monoclonal antibodies. It is also used to detect chimeric antigen receptor (CAR) expressing cells.
Product Details
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
- Application Notes
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For multi-color flow cytometry analysis, cells should be stained with Protein L first, and washed; costaining reagent(s) need to be applied as the second step.
Antigen Details
- Cell Type
- B cells
- Biology Area
- Cell Biology, Immuno-Oncology, Immunology
- Antigen References
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- Björck L. 1988. J Immunol. 140:1194-7.
- Akerström B and Björck L. 1989. J Biol Chem. 264:19740-6.
- Gene ID
- NA
- UniProt
- View information about Protein L on UniProt.org