- Regulatory Status
- RUO
- Other Names
- Phalloidin
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HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes then permeabilized with 0.5% Triton X-100 for ten minutes and blocked by 5% FBS for 30 minutes. Then, the cells were stained with Flash Phalloidin™ Red 594 (5 µl of the stock solution in 200 µL of 5% FBS) for 20 minutes at 4°C in the dark. The nuclei were then counterstained with DAPI (blue). -
C57 mouse frozen cerebellum tissue was fixed with 4% paraformaldehyde (PFA) for ten minutes then permeabilized with 0.5% Triton X-100 for ten minutes and blocked by 5% FBS for 30 minutes. Then, the tissue was stained with Flash Phalloidin™ Red 594 (red, 25 µL of the stock solution in 1 mL of PBS) for 30 minutes at 4°C in the dark. The tissue was then counterstained with DAPI (blue). The image was captured by 20X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 424203 | 300 units | $347.00 | |||
Molecular Mass 1535.79 g/mol. Flash Phalloidin™ Red 594 excites maximally at 590 nm and emits maximally at 611 nm.
Phalloidin is a bicyclic peptide that can be found naturally in the death cap mushroom. This molecule is considered to bind so tightly to F-actin that when ingested by an organism, it will prevent the depolymerization of the actin polymeric filaments which leads to cellular toxicity. In cell imaging, this is a very useful probe for imaging and stabilizing filamentous F-actin in fixed and permeabilized cells, providing structural and volumetric context to the cell. Phallotoxins are conjugated to a wide array of fluorophores to enable their use in multicolor microscopy.
Product Details
- Verified Reactivity
- Human, Mouse, Rat, All Species
- Preparation
- Flash Phalloidin™ Red 594 is lyophilized. Reconstitute with 1.5mL of methanol to make a stock solution of 300 units.
- Storage & Handling
- Store Flash Phalloidin™ Red 594 at -20°C, protected from light.
- Application
-
ICC - Quality tested
IHC-F - Verified - Recommended Usage
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Reconstitute the Flash Phalloidin™ Red 594 with 1.5mL of 100% methanol to make 300 units. Then prepare a working concentration by diluting 1:20 - 1:100 of stock in 1X PBS. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
- Prior to reconstitution, spin down the vial of lyophilized reagent in a microcentrofuge to ensure the reagent is at the bottom of the vial.
- Fix cultured cells with 1% - 4% paraformaldehyde (PFA) for 10 minutes at room temperature.
- Wash the cells two times with 1X PBS.
- Permeabilize the cells with 0.5% Triton X-100 for 10 minutes at room temperature or at 4°C.
- Wash the cells two times with 1X PBS.
- Block cells with 5% fetal bovine serum for 30 minutes at room temperature.
- Prepare the working solution by diluting Flash Phalloidin™ Red 594 1:20 - 1:100 in 1X PBS.
- Stain the cells with diluted solution for 20 minutes at room temperature in the dark protected from light.
- Mount the slides with an antifade mounting media and image the slides.
Antigen Details
- Distribution
-
Cytoskeleton.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience
- Gene ID
- NA