GMP APC anti-human IFN-γ Antibody

Pricing & Availability
Clone
4S.B3 (See other available formats)
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Mouse IgG1, κ
4SdotB3_GMP_APC_IFNgamma_Antibody_082024
Typical result of PMA + Ionomycin with Brefeldin A stimulated (4-hour) human peripheral blood lymphocytes fixed with Fixation Buffer (Cat# 420801), permeabilized with Permeabilization Wash Buffer (Cat# 421002), and intracellularly stained either with 4S.B3 APC used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
  • 4SdotB3_GMP_APC_IFNgamma_Antibody_082024
    Typical result of PMA + Ionomycin with Brefeldin A stimulated (4-hour) human peripheral blood lymphocytes fixed with Fixation Buffer (Cat# 420801), permeabilized with Permeabilization Wash Buffer (Cat# 421002), and intracellularly stained either with 4S.B3 APC used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
Compare all formats See GMP APC spectral data
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260372 100 tests $347.00
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Technical data sheet

Product Details

Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partially purified, native human IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
25 µg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA or ELISPOT Detection5: The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.
Flow Cytometry3,4,6-8: The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.
Additional reported applications (for the relevant formats) include: neutralization1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.
Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Meager A, et al. 1984. J. Interferon Res. 4:619. (Neut)
  2. Meager A, 1987. Lymphokines and Interferons:A Practical Approach. IRL Press Ltd, Oxford, p. 105. (Neut)
  3. Sester M, et al. 2002. J. Virol. 76:3748. (ICFC)
  4. Infante-Duarte C, et al. 2000 J. Immunol. 165:6107. (ICFC)
  5. Goodier M, et al. 2000. J. Immunol. 165:139. (ELISA)
  6. Chen H, et al. 2005. J. Immunol. 175:591. (ICFC)
  7. Smeltz RB, 2007. J. Immunol. 178:4786. (ICFC)
  8. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (ICFC)
Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

Antigen Details

Structure
Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Other Formats

View All IFN-γ Reagents Request Custom Conjugation
Description Clone Applications
PE anti-human IFN-γ 4S.B3 ICFC
APC anti-human IFN-γ 4S.B3 ICFC
FITC anti-human IFN-γ 4S.B3 ICFC
Biotin anti-human IFN-γ 4S.B3 ELISA Detection,ELISPOT Detection,ICFC
Purified anti-human IFN-γ 4S.B3 ELISA,WB,ICFC
Alexa Fluor® 488 anti-human IFN-γ 4S.B3 ICFC
Alexa Fluor® 647 anti-human IFN-γ 4S.B3 ICFC
Alexa Fluor® 700 anti-human IFN-γ 4S.B3 ICFC
Pacific Blue™ anti-human IFN-γ 4S.B3 ICFC
PerCP/Cyanine5.5 anti-human IFN-γ 4S.B3 ICFC
APC/Cyanine7 anti-human IFN-γ 4S.B3 ICFC
PE/Cyanine7 anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 421™ anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 570™ anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 605™ anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 650™ anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 711™ anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 785™ anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 510™ anti-human IFN-γ 4S.B3 ICFC
PE/Dazzle™ 594 anti-human IFN-γ 4S.B3 ICFC
APC/Fire™ 750 anti-human IFN-γ 4S.B3 ICFC
PerCP anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 750™ anti-human IFN-γ 4S.B3 ICFC
KIRAVIA Blue 520™ anti-human IFN-γ Antibody 4S.B3 ICFC
Spark NIR™ 685 anti-human IFN-γ Antibody 4S.B3 ICFC
APC anti-human IFN-γ 4S.B3 ICFC
Spark Blue™ 515 anti-human IFN-γ 4S.B3 ICFC
Spark UV™ 387 anti-human IFN-γ 4S.B3 ICFC
Spark PLUS UV395™ anti-human IFN-γ 4S.B3 ICFC
PE anti-human IFN-γ 4S.B3 ICFC
GMP APC anti-human IFN-γ 4S.B3 ICFC
Go To Top Version: 1    Revision Date: 09/17/2024

For research use only. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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