GMP PE anti-human CD11b Antibody

Pricing & Availability
Clone
ICRF44 (See other available formats)
Workshop
IV M047
Other Names
Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM
Isotype
Mouse IgG1, κ
ICRF44_GMP_PE_CD11b_Antibody_022222
Typical results from human peripheral blood granulocytes stained either with ICRF44 PE used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
  • ICRF44_GMP_PE_CD11b_Antibody_022222
    Typical results from human peripheral blood granulocytes stained either with ICRF44 PE used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
Compare all formats See GMP PE spectral data
Cat # Size Price Quantity Check Availability
260042 100 tests $305.00
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Description

CD11b is a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, and factor X.

Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
200 µg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy5, stimulation of monocytes3, blocking of heterotypic PMN aggregation8, and blocking of granulocyte activation12. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301361 & 301362).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  2. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  3. Rezzonico R, et al. 2001. Blood 97:2932. (Stim)
  4. Marsik C, et al. 2003. Shock 20:493. (FC)
  5. David A, et al. 2003. J. Leukoc. Biol. 74:551. (IF)
  6. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  7. Thurlow LR, et al. 2010. Infect. Immun. 128:1128. (FC) PubMed
  8. Jadhav S, et al. 2001. J. Immunol. 167:5986. (Block)
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  10. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. (FC)
  11. Wen T, et al. 2014. J Immunol. 192:5481. (FC) PubMed
  12. Sprong T, et al. 2003. Blood 102:3702. (Block)
  13. Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
  14. Larsson K, et al. 2015. PNAS. PubMed
Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:​​

  • Batch-to-batch consistency
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 165-170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subset of T cells, subset of B cells

Function
Adhesion, phagocytosis, chemotaxis, neutrophil activation
Ligand/Receptor
ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Stewart M, et al. 1995. Curr Opin Cell Biol. 7:690-6.
Gene ID
3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

Other Formats

View All CD11b Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD11b ICRF44 FC
Biotin anti-human CD11b ICRF44 FC
PE anti-human CD11b ICRF44 FC
PE/Cyanine5 anti-human CD11b ICRF44 FC
Purified anti-human CD11b ICRF44 FC,CyTOF®,ICC,IHC
Pacific Blue™ anti-human CD11b ICRF44 FC
Alexa Fluor® 488 anti-human CD11b ICRF44 FC
Alexa Fluor® 647 anti-human CD11b ICRF44 FC
PE/Cyanine7 anti-human CD11b ICRF44 FC
PerCP/Cyanine5.5 anti-human CD11b ICRF44 FC
Brilliant Violet 421™ anti-human CD11b ICRF44 FC,ICC
Brilliant Violet 570™ anti-human CD11b ICRF44 FC
FITC anti-human CD11b ICRF44 FC
Brilliant Violet 605™ anti-human CD11b ICRF44 FC
Brilliant Violet 510™ anti-human CD11b ICRF44 FC,ICC
Brilliant Violet 650™ anti-human CD11b ICRF44 FC
Purified anti-human CD11b (Maxpar® Ready) ICRF44 FC,CyTOF®
Alexa Fluor® 594 anti-human CD11b ICRF44 ICC,FC
APC/Cyanine7 anti-human CD11b ICRF44 FC
Brilliant Violet 711™ anti-human CD11b ICRF44 FC
Brilliant Violet 785™ anti-human CD11b ICRF44 FC
PE/Dazzle™ 594 anti-human CD11b ICRF44 FC
APC/Fire™ 750 anti-human CD11b ICRF44 FC
APC anti-human CD11b ICRF44 FC
PE anti-human CD11b ICRF44 FC
TotalSeq™-A0161 anti-human CD11b ICRF44 PG
Alexa Fluor® 700 anti-human CD11b ICRF44 FC
PE/Cyanine7 anti-human CD11b ICRF44 FC
TotalSeq™-B0161 anti-human CD11b ICRF44 PG
TotalSeq™-C0161 anti-human CD11b ICRF44 PG
PerCP/Cyanine5.5 anti-human CD11b ICRF44 FC
Ultra-LEAF™ Purified anti-human CD11b ICRF44 FC,CyTOF®,Block,ICC,IHC
TotalSeq™-D0161 anti-human CD11b ICRF44 PG
GMP PE/Cyanine7 anti-human CD11b ICRF44 FC
Pacific Blue™ anti-human CD11b ICRF44 FC
GMP PE anti-human CD11b ICRF44 FC
Spark UV™ 387 anti-human CD11b ICRF44 FC
GMP PerCP/Cyanine5.5 anti-human CD11b ICRF44 FC
FITC anti-human CD11b ICRF44 FC
APC/Fire™ 750 anti-human CD11b ICRF44 FC
GMP APC anti-human CD11b ICRF44 FC
GMP FITC anti-human CD11b ICRF44 FC
GMP APC/Fire™ 750 anti-human CD11b ICRF44 FC
Spark Blue™ 515 anti-human CD11b ICRF44 FC
Spark Violet™ 500 anti-human CD11b ICRF44 FC
Spark Red™ 718 anti-human CD11b (Flexi-Fluor™) ICRF44 FC
Go To Top Version: 1    Revision Date: 02/22/2022

For research use only. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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