GMP PE/Dazzle™ 594 anti-human CD64 Antibody

Pricing & Availability
Clone
10.1 (See other available formats)
Workshop
VI MA36
Other Names
FcγRI, FcR I
Isotype
Mouse IgG1, κ
10dot1_GMP-PEDazzle594_CD64_Antibody_030923
Typical results from human peripheral blood monocytes stained either with 10.1 PE/Dazzle™ 594 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
  • 10dot1_GMP-PEDazzle594_CD64_Antibody_030923
    Typical results from human peripheral blood monocytes stained either with 10.1 PE/Dazzle™ 594 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
Compare all formats See GMP PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Check Availability
260248 100 tests $452.00
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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
Formulation
Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
100 µg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections12.

Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874.
  3. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  4. Holl V, et al. 2004. J. Immunol. 173:6274.
  5. Hober D, et al. 2002. J. Gen. Virol. 83:2169.
  6. Cho HJ, et al. 2007. Physiol Genomics 149:60.
  7. van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed
  8. Bruhns P, et al. 2008. Blood 113:3716. PubMed
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  10. Carter DL, et al. 1999. Cytometry 37:41. (FC)
  11. Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453.
  12. Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)
Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, dendritic cells, activated granulocytes

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG receptor
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Hulett M, et al. 1994. Adv. Immunol. 57:1.
2. van de Winkel J, et al. 1993. Immunol. Today 14:215.

Gene ID
2209 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org

Other Formats

View All CD64 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-human CD64 10.1 FC
FITC anti-human CD64 10.1 FC
PE anti-human CD64 10.1 FC
Purified anti-human CD64 10.1 FC,IHC-F,Block
Alexa Fluor® 488 anti-human CD64 10.1 FC
Alexa Fluor® 647 anti-human CD64 10.1 FC
APC anti-human CD64 10.1 FC
Pacific Blue™ anti-human CD64 10.1 FC
Brilliant Violet 421™ anti-human CD64 10.1 FC
PE/Cyanine7 anti-human CD64 10.1 FC
PerCP/Cyanine5.5 anti-human CD64 10.1 FC
APC/Cyanine7 anti-human CD64 10.1 FC
Brilliant Violet 510™ anti-human CD64 10.1 FC
Purified anti-human CD64 (Maxpar® Ready) 10.1 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD64 10.1 FC
Brilliant Violet 605™ anti-human CD64 10.1 FC
APC/Fire™ 750 anti-human CD64 10.1 FC
PE anti-human CD64 10.1 FC
PE/Dazzle™ 594 anti-human CD64 10.1 FC
FITC anti-human CD64 10.1 FC
TotalSeq™-A0162 anti-human CD64 10.1 PG
Brilliant Violet 711™ anti-human CD64 10.1 FC
Alexa Fluor® 700 anti-human CD64 10.1 FC
Brilliant Violet 785™ anti-human CD64 10.1 FC
TotalSeq™-C0162 anti-human CD64 10.1 PG
Ultra-LEAF™ Purified anti-human CD64 10.1 FC,IHC-F,Block
TotalSeq™-B0162 anti-human CD64 10.1 PG
TotalSeq™-D0162 anti-human CD64 10.1 PG
GMP PE anti-human CD64 10.1 FC
GMP FITC anti-human CD64 10.1 FC
Brilliant Violet 650™ anti-human CD64 10.1 FC
GMP PE/Dazzle™ 594 anti-human CD64 10.1 FC
PE/Cyanine7 anti-human CD64 10.1 FC
APC/Fire™ 750 anti-human CD64 10.1 FC
PerCP/Cyanine5.5 anti-human CD64 10.1 FC
Pacific Blue™ anti-human CD64 10.1 FC
GMP APC/Fire™ 750 anti-human CD64 10.1 FC
GMP PE/Cyanine7 anti-human CD64 10.1 FC
Go To Top Version: 1    Revision Date: 03/17/2023

For research use only. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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