- Clone
- 11A50-B10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
- Previously
-
Signet Catalog# 9146-02
Covance Catalog# SIG-39146
- Isotype
- Mouse IgG1, κ
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IHC staining of HRP anti-β-Amyloid, 1-40 antibody (clone 11A50-B10) on formalin-fixed paraffin-embedded Alzheimer’s disease human brain tissue. Following antigen retrieval using 88% formic acid for 20 minutes at room temperature, the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of HRP anti-β-Amyloid, 1-40 antibody (clone 11A50-B10) on formalin-fixed paraffin-embedded Alzheimer’s disease human brain tissue. Following antigen retrieval using 88% formic acid for 20 minutes at room temperature, the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 805407 | 200 µL | $633.00 | |||
Amyloid beta (Aβ or Amyloid beta) denotes peptides of 36-43 amino acids in length that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. The peptides result from the amyloid precursor protein (APP), which is cut by certain enzymes to yield Aβ. Aβ molecules can aggregate to form oligomers (known as "seeds") which are believed to be able to induce other Aβ molecules to also take the misfolded oligomeric form, leading to a chain reaction akin to a prion infection. The seeds or the resulting amyloid plaques are toxic to nerve cells. The other protein implicated in Alzheimer's disease, tau protein, also forms such prion-like misfolded oligomers, and there is some evidence that misfolded Aβ can induce tau to misfold.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light. For storage of this antibody when diluted below a concentration of 50 µg/mL, a carrier protein such as 2% bovine serum albumin is required.
- Application
-
IHC-P - Quality tested
Dot Blot, ELISA, IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone 11A50-B10 is reactive to the C-terminus of beta amyloid and is specific for the isoform ending at the 40th amino acid.
-
Application References
(PubMed link indicates BioLegend citation) -
- Watts JC, et al. 2014. Proc. Natl. Sci. USA 28:10323. (IHC-P) PubMed
- Diomede L, et al. 2014. Neurobiol. Dis. 62:521. (Dot Blot) PubMed
- Watanabe H, et al. 2012. J Neurosci. 15:5085. (ELISA) PubMed
- Portelius E, et al. 2006. J. Proteome Res. 4:1010. (IP)
- Vingtdeux V, et al. 2011. FASEB J. 25:219. (ELISA) PubMed
- Ohmi K, et al. 2011. PLoS One 11:e27461. (IHC-Other) PubMed
- Product Citations
-
- RRID
-
AB_2564979 (BioLegend Cat. No. 805407)
Antigen Details
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- APP/β-Amyloid
- Gene ID
- 351 View all products for this Gene ID
- UniProt
- View information about beta-Amyloid 1-40 on UniProt.org
Other Formats
View All β-Amyloid, 1-40 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-β-Amyloid, 1-40 | 11A50-B10 | ELISA,WB,IHC,IP |
| HRP anti-β-Amyloid, 1-40 | 11A50-B10 | IHC-P,Dot Blot,ELISA,IP |
| Purified anti-β-Amyloid, 1-40 | 11A50-B10 | IHC-P,Dot Blot,ELISA |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-β-Amyloid, 1-40
-
HRP anti-β-Amyloid, 1-40
IHC staining of HRP anti-β-Amyloid, 1-40 antibody (clone 11A... 
IHC staining of HRP anti-β-Amyloid, 1-40 antibody (clone 11A... -
Purified anti-β-Amyloid, 1-40
IHC staining of purified anti-β-Amyloid, 1-40 antibody (clon... 
IHC staining of purified anti-β-Amyloid, 1-40 antibody (clon...