HRP anti-β-Amyloid, 1-42 Antibody (Previously Covance catalog# SIG-39147)

Pricing & Availability
Clone
12F4 (See other available formats)
Regulatory Status
RUO
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Signet Catalog# 9147-02
Covance Catalog# SIG-39147
Isotype
Mouse IgG1, κ
12F4_HRP_bAmyloid_1-42_Antibody_1_081518
IHC staining of HRP anti-β-Amyloid, 1-42 antibody (clone 12F4) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 12F4_HRP_bAmyloid_1-42_Antibody_1_081518
    IHC staining of HRP anti-β-Amyloid, 1-42 antibody (clone 12F4) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 12F4_HRP_bAmyloid_1-42_Antibody_2_081518
    Western blot of HRP anti-β-Amyloid, 1-42 antibody (clone 12F4). Lane 1: 10 µg Human Aβ 1-40 peptide; Lane 2: 10 µg Human Aβ 1-42 peptide. Lane 3: 500 ng Recombinant human 751 protein; Lane 4: 10 µg Rodent Aβ 1-40 peptide; Lane 5: 10 µg Rodent Aβ 1-42 peptide. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
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805507 200 µL $633.00
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Description

Amyloid beta (Aβ or Amyloid beta) denotes peptides of 36-43 amino acids in length that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. The peptides result from the amyloid precursor protein (APP), which is cut by certain enzymes to yield Aβ. Aβ molecules can aggregate to form oligomers (known as "seeds") which are believed to be able to induce other Aβ molecules to also take the misfolded oligomeric form, leading to a chain reaction akin to a prion infection. The seeds or the resulting amyloid plaques are toxic to nerve cells. The other protein implicated in Alzheimer's disease, tau protein, also forms such prion-like misfolded oligomers, and there is some evidence that misfolded Aβ can induce tau to misfold.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in ELISA, Immunoblotting (WB) and Immunohistochemistry on frozen and formalin-fixed paraffin-embedded tissues (IHC-P, IHC-F).

Reactive to the c-terminus of beta amyloid and specific for the isoform ending at the 42nd amino acid.

Additional reported applications (for the relevant formats) includes: immunohistochemical staining of paraformaldehyde-fixed frozen sections6 and ELISA3,4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Helwig M, et al. 2013. J. Biol. Chem. 288:1114. (IHC-P)
  2. Takami M, et al.2009. J. Neurosci.; 29: 13042. (IHC-P)
  3. Cenini G, et al. 2012. Biochim Biophys Acta. 1822(2):130. (ELISA) PubMed
  4. Sundaram RK, et al. 2012. Int. J. Pept. Res. Ther. 18(2):99. (ELISA) PubMed
  5. Savage MJ, et al. 2014. J. Neurosci. 8:2884. (ELISA) PubMed
  6. Mastrangelo MA, Bowers WJ. 2008. BMC Neurosci. 9:81. (IHC-F) PubMed
Product Citations
  1. Liu X, et al. 2023. Clin Transl Med. e1250:13. PubMed
  2. Casali BT, et al. 2020. Neurobiol Dis. 142:104956. PubMed
  3. Cheng-Hathaway PJ, et al. 2018. Mol Neurodegener. 13:e0200060. PubMed
RRID
AB_2564980 (BioLegend Cat. No. 805507)

Antigen Details

Structure
Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution

Tissue sources: Primarily nervous system, but also adipose tissue, intestine, muscle. 
Distribution: cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Antigen References
  1. Kumar A, et al. 2015. Pharmacol. Rep. 67(2):195.
  2. Sadigh-Eteghad S, et al. 2015. Med. Princ. Pract. 24(1):1
  3. Hampel H, et al. 2015. Expert Rev. Neurother. 15(1):83.
  4. Puig KL, et al. 2012.  Exp. Gerontol. 48(7): 608.
  5. Selkoe DJ, et al. 2016. EMBO Mol. Med. 8(6):595.
  6. Walsh DM, et al.  2007. J. Neurochem. 101(5):1172.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid 1-42 on UniProt.org
Go To Top Version: 6    Revision Date: 02/13/2024

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