HRP Donkey anti-human IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly24109 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
Cat # Size Price Quantity Check Availability
410902 500 µL $141.00
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Description

This polyclonal donkey anti-human IgG antibody reacts with the heavy chains of human IgG and with the light (kappa and lambda) chains common to most human immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, bovine, horse, and rabbit immunoglobulins, but it may cross reaction with other subclasses of human immunoglobulins.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Aqueous buffered solution containing a carrier protein and preservatives.
Preparation
The polyclonal antibody was purified from donkey antiserum by human immunoglobulin affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA. Do not use this product beyond the expiration date. For ELISA, the reagent should be titrated between 1:1,000 - 1:20,000 to determine optimal conditions. Avoid using diluent solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase.

Application Notes

Cross-reactivity with the light chains of other human immunoglobulins, e.g. IgE and IgM.

Product Citations
  1. Tsai DY, et al. 2023. Vaccines (Basel). 11:. PubMed
  2. Williams DM, et al. 2023. iScience. 26:107056. PubMed
  3. Sun X, et al. 2022. Appl Environ Microbiol. 88:e0230321. PubMed
  4. Biering SB, et al. 2022. Nat Commun. 13:7630. PubMed
  5. Escobar A, et al. 2022. Front Immunol. 12:766278. PubMed
  6. Bosshard N, et al. 2021. Rheumatol Ther. 8:1043. PubMed
  7. Gonzales SJ, et al. 2022. Front Immunol. 13:809264. PubMed
RRID
AB_2686937 (BioLegend Cat. No. 410902)
Go To Top Version: 0    Revision Date: 04/13/2017

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  • HRP Donkey anti-human IgG (minimal x-reactivity)

    Poly24109_HRP_IgG_Antibody_040517
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