LDH-Cytox™ Assay Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Lactate dehydrogenase assay
LDH_Cytox_Assay_Kit_Large_082117
Human FASL induced cytotoxicity in Jurkat cells is measured using both LDH-Cytox™ Assay Kit (OD 490nm) and Deep Blue Cell Viability™ Kit (RFU x 103).
  • LDH_Cytox_Assay_Kit_Large_082117
    Human FASL induced cytotoxicity in Jurkat cells is measured using both LDH-Cytox™ Assay Kit (OD 490nm) and Deep Blue Cell Viability™ Kit (RFU x 103).
Cat # Size Price Quantity Check Availability
426401 500 tests $229.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

LDH-Cytox Assay™ Kit is a kit for determination of cytotoxicity by measuring lactate dehydrogenase activity released from damaged cells. LDH catalyzes dehydrogenation of lactate to pyruvate thereby reducing NAD+ to NADH.  NADH reduces water-soluble tetrazolium salt in the presence of an electron mediator to produce an orange formazan dye.

Technical data sheet

Kit Contents

Kit Contents
  • Dye Mixture: Lyophilized, 1 bottle
  • Assay Buffer: 55 mL
  • Lysis Buffer: 5.5 mL
  • Stop Solution: 27.5 mL

Product Details

Formulation
This solution contains WST.
Application

Cyt - Quality tested
FA, BA - Reported in the literature, not verified in house

Recommended Usage

For more information, please refer to the product manual.

Application References

(PubMed link indicates BioLegend citation)
  1. Jin SF, et al. 2015. Exp Cell Res. 339(2):289-99.
  2. Watanabe S, et al. 2016. Biochem Biophys Res Commun. 471:191.
  3. Wu L, et al. 2015. Am J Physiol Gastrointest Liver Physiol. 309:G695.
  4. Fukami T, et al. 2016. Biochem. Pharmacol. 116:153-61.
  5. Tanaka M, et al. 2016. J Pharmacol Sci. 131(3):162-71.
  6. Wakatsuki S, et al. 2015. J Cell Biol. 211(4):881-96.
Product Citations
  1. Arimoto KI, et al. 2023. Nat Commun. 14:251. PubMed
  2. Lyu Z, et al. 2021. Nat Biomed Eng. 5:847. PubMed
  3. Seyed-Khorrami SM, et al. 2021. Cancer Cell Int. 21:244. PubMed
  4. Hao Q, et al. 2022. Cells. 11:. PubMed
  5. Camp FA, et al. 2022. Cancers (Basel). 14:. PubMed
  6. Sauler M, et al. 2022. Nat Commun. 13:494. PubMed

Antigen Details

Biology Area
Cell Biology, Cell Proliferation and Viability, Mitochondrial Function, Signal Transduction
Gene ID
NA
Go To Top Version: 3    Revision Date: 05/20/2020

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login/Register
Forgot your password? Reset Password
Request an Account