- Clone
- W19242A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-18 receptor alpha chain, IL-18 receptor 1, IL-18 receptor related protein, CDw218a
- Isotype
- Rat IgG2a, κ
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Recombinant human IL-18 (Cat. No. 592104) (black circles) stimulates the production of human IFN-γ (Cat. No. 430104) in human KG-1 cells. LEAF™ purified anti-human CD218a (IL-18Rα) (clone W19242A) (purple squares) inhibits this effect of recombinant human IL-18 (Cat. No. 592104) at 8 ng/mL in KG-1 cells in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have this effect. ND50 range: 1-7 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 601253 | 100 µg | $430.00 | |||
| 601254 | 1 mg | $1115.00 | |||
IL-18 is essential for both innate and adaptive immunity. The IL-18 receptor is composed of an α and a β subunit that form a high affinity heterodimer for IL-18. IL-18R1, also known as IL-18Rα chain and CD218a, is a type I transmembrane protein. It is expressed on NK cells, neutrophils, endothelial cells, and subsets of T and B cells. The expression of IL-18R1 and IL-18RAcP (IL-18Rβ) on lymphocytes are upregulated upon IL-12 stimulation. IL-23 and IL-18 synergistically enhance IL-18R1 expression on NK-like T cells. Upon IL-18 engagement, the intracellular TIR domain of IL-18R1 and IL-18RAcP recruits adaptor proteins, MyD88, and in turn recruits IRAK and TRAF6, activating NF-κB and MAPK pathways, which upregulates production of various inflammatory cytokines. The interaction of IL-18 and IL-18 receptor has been reported to be implicated in promotion of Th1 cytokine production and atherogenesis. IL-37 also binds to IL-18R1, but recruits IL-1R8 (SIGIRR) to activate anti-inflammatory responses.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human CD218a (IL-18Rα)
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
- Application
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Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by neutralizing the production of human IFN-γ induced by recombinant human IL-18 at 8 ng/mL in KG-1 cells. ND50 range: 1-7 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_3097579 (BioLegend Cat. No. 601253)
AB_3097579 (BioLegend Cat. No. 601254)
Antigen Details
- Structure
- Disulfide-linked homodimer
- Distribution
-
Expressed on NK cells and neutrophils, IL-12 activated T and B cells, endothelial cells, and smooth muscle cells; highly expressed on Hodgkin's disease cell lines
- Function
- IL-18Rα forms a receptor complex with IL-18Rβ and mediates IL-18 function
- Interaction
- Human IL-18Rα inhibits the induction of IFN-γ by human IL-18 in human KG-1 cells
- Ligand/Receptor
- IL-18
- Cell Type
- B cells, Endothelial cells, Neutrophils, NK cells, T cells
- Biology Area
- Adaptive Immunity, Immunology, Signal Transduction
- Molecular Family
- Cytokine/Chemokine Receptors
- Antigen References
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- Torigoe K, et al. 1997. J Biol Chem. 272:25737-42.
- Born TL, et al. 1998. J Biol Chem. 273:29445-50.
- Airoldi I, et al. 2000. J Immunol. 165:6880-8.
- van de Wetering D, et al. 2008. Int Immunol. 21:145-53.
- Adachi O, et al. 1998. Immunity. 9:143-50.
- Tsutsumi N, et al. 2014. Nat Commun. 5:5340.
- Gene ID
- 8809 View all products for this Gene ID
- UniProt
- View information about CD218a on UniProt.org
Other Formats
View All CD218a Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| LEAF™ Purified anti-human CD218a (IL-18Rα) | W19242A | Neut |
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LEAF™ Purified anti-human CD218a (IL-18Rα)
Recombinant human IL-18 (Cat. No. 592104) (black circles) st...